Action of mycocins produced by Wickerhamomyces anomalus on Malassezia pachydermatis isolated from the ear canal of dogs.

Autor: da Silva JC; Hospital Universitário, Universidade Estadual do Oeste do Paraná, Cascavel, PR, Brazil., Cerny MG; Hospital Universitário, Universidade Estadual do Oeste do Paraná, Cascavel, PR, Brazil., Nascimento BL; Hospital Universitário, Universidade Estadual do Oeste do Paraná, Cascavel, PR, Brazil., Martelli EC; Hospital Universitário, Universidade Estadual do Oeste do Paraná, Cascavel, PR, Brazil., Vieira J; Hospital Universitário, Universidade Estadual do Oeste do Paraná, Cascavel, PR, Brazil., de Souza Marquezoni R; Hospital Universitário, Universidade Estadual do Oeste do Paraná, Cascavel, PR, Brazil., Makita MT; Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Rio de Janeiro, RJ, Brazil., Paula CR; Faculdade de Odontologia, Universidade de São Paulo, São Paulo, SP, Brazil., de Assis Baroni F; Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Rio de Janeiro, RJ, Brazil., Gandra RF; Hospital Universitário, Universidade Estadual do Oeste do Paraná, Cascavel, PR, Brazil. Electronic address: rinaldo.gandra@unioeste.br.
Jazyk: angličtina
Zdroj: Research in veterinary science [Res Vet Sci] 2024 Aug; Vol. 176, pp. 105319. Date of Electronic Publication: 2024 May 31.
DOI: 10.1016/j.rvsc.2024.105319
Abstrakt: This study aims to examine the effectiveness of mycocins produced by Wickerhamomyces anomalus in inhibiting Malassezia pachydermatis, a yeast commonly found in the ear canal of dogs. M. pachydermatis has a zoophilic origin and can be found in mammals, and frequently in dogs, where it mainly colonizes the ear canal region and the skin, leading to lesions that are difficult to treat. The antimicrobial mechanism was evaluated using dilutions of supernatant with enzymatic activity, which may include β-glucanases, glycoproteins known to act on microorganism cell walls. However, it is important to note that this supernatant may contain other compounds as well. β-glucanases in the mycocins supernatant were found at a concentration of 0.8 U/mg. The susceptibility of M. pachydermatis isolates was tested using the microdilution method. The isolates suffered 100% inhibition when tested with the culture supernatant containing mycocins. In the proteinases production test, 44% of the isolates tested were strong proteinases producers. Subsequently all these isolates suffered inhibition of their activity when tested in research medium containing mycocins supernatant at a subinhibitory concentration of β-glucanases. This shows that mycocins can inhibit the production of proteinases, a virulence factor of M. pachydermatis. The viability test showed the antifungal action of mycocins in inhibiting the viability of M. pachydermatis cells after a period of 8  hours of contact. These results support the antimicrobial potential of mycocins and their promise as a therapeutic option.
Competing Interests: Declaration of competing interest None.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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