Performance Evaluation of a Commercial Automated Library Preparation System for Clinical Microbial Whole-Genome Sequencing Assays.

Autor:	Caldera JR; Department of Pathology and Laboratory Medicine, UCLA Health and David Geffen School of Medicine, Los Angeles, California., Anikst V; Department of Pathology and Laboratory Medicine, UCLA Health and David Geffen School of Medicine, Los Angeles, California., Gray H; Department of Pathology and Laboratory Medicine, UCLA Health and David Geffen School of Medicine, Los Angeles, California., Tsan A; Department of Pathology and Laboratory Medicine, UCLA Health and David Geffen School of Medicine, Los Angeles, California., Sono R; Department of Pathology and Laboratory Medicine, UCLA Health and David Geffen School of Medicine, Los Angeles, California., Yang S; Department of Pathology and Laboratory Medicine, UCLA Health and David Geffen School of Medicine, Los Angeles, California. Electronic address: shangxinyang@mednet.ucla.edu.
Jazyk:	angličtina
Zdroj:	The Journal of molecular diagnostics : JMD [J Mol Diagn] 2024 Aug; Vol. 26 (8), pp. 719-726. Date of Electronic Publication: 2024 Jun 06.
DOI:	10.1016/j.jmoldx.2024.05.006
Abstrakt:	Next-generation sequencing (NGS) has proven clinical utility on disease management and serves as an important tool for genomic surveillance. Currently, hurdles surrounding its implementation, namely the complex and demanding analytical workflows, have impeded its widespread use in many laboratories. To address this challenge, the UCLA Molecular Microbiology and Pathogen Genomics Laboratory evaluated the performance of the Tecan MagicPrep NGS system, a commercial automated solution for library preparation for clinical whole-genome sequencing assays, against the Illumina Nextera DNA Flex Library Prep. Using 35 unique organisms (28 bacteria and 7 fungi) for various clinical applications, including microbial identification and genomic characterization, we compared the quantity and quality of the prepared libraries and the resulting sequences, and concordance of the overall results. We also assessed the impact of its implementation on laboratory workflow. The MagicPrep NGS produced higher library concentrations with smaller sizes, and correspondingly, higher molarity. Quality metrics of the sequences, however, demonstrated no significant impact on the overall results, producing 100% concordance with the reference method. Importantly, workflow analysis showed 5 hours less hands-on time per run with more flexibility. This evaluation study indicates that performance of the MagicPrep NGS is comparable to the Nextera DNA Flex with the added benefit of improving workflow efficiency and reducing labor for performing routine clinical microbial whole-genome sequencing tests. Competing Interests: Disclosure Statement None declared. (Copyright © 2024 Association for Molecular Pathology and American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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