[n]Cycloparaphenylenes as Compatible Fluorophores for Melt Electrowriting.

Autor: Hall PC; Phil and Penny Knight Campus for Accelerating Scientific Impact, University of Oregon, 1505 Franklin Boulevard, Eugene, OR, 97403-6231, USA., Reid HW; Department of Chemistry and Biochemistry & Materials Science Institute, University of Oregon, 1505 Franklin Boulevard, Eugene, OR, 97403-6231, USA., Liashenko I; Phil and Penny Knight Campus for Accelerating Scientific Impact, University of Oregon, 1505 Franklin Boulevard, Eugene, OR, 97403-6231, USA., Tandon B; Phil and Penny Knight Campus for Accelerating Scientific Impact, University of Oregon, 1505 Franklin Boulevard, Eugene, OR, 97403-6231, USA.; Microsystems Laboratory, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, CH-1015, Switzerland., O'Neill KL; Phil and Penny Knight Campus for Accelerating Scientific Impact, University of Oregon, 1505 Franklin Boulevard, Eugene, OR, 97403-6231, USA., Paxton NC; Phil and Penny Knight Campus for Accelerating Scientific Impact, University of Oregon, 1505 Franklin Boulevard, Eugene, OR, 97403-6231, USA.; Centre for Biomedical Technologies (CBT), Queensland University of Technology (QUT), 2 George St, Brisbane, QLD, 4000, Australia., Lindberg GCJ; Phil and Penny Knight Campus for Accelerating Scientific Impact, University of Oregon, 1505 Franklin Boulevard, Eugene, OR, 97403-6231, USA., Jasti R; Phil and Penny Knight Campus for Accelerating Scientific Impact, University of Oregon, 1505 Franklin Boulevard, Eugene, OR, 97403-6231, USA.; Department of Chemistry and Biochemistry & Materials Science Institute, University of Oregon, 1505 Franklin Boulevard, Eugene, OR, 97403-6231, USA., Dalton PD; Phil and Penny Knight Campus for Accelerating Scientific Impact, University of Oregon, 1505 Franklin Boulevard, Eugene, OR, 97403-6231, USA.
Jazyk: angličtina
Zdroj: Small (Weinheim an der Bergstrasse, Germany) [Small] 2024 Oct; Vol. 20 (40), pp. e2400882. Date of Electronic Publication: 2024 Jun 06.
DOI: 10.1002/smll.202400882
Abstrakt: Fluorescent probes are an indispensable tool in the realm of bioimaging technologies, providing valuable insights into the assessment of biomaterial integrity and structural properties. However, incorporating fluorophores into scaffolds made from melt electrowriting (MEW) poses a challenge due to the sustained, elevated temperatures that this processing technique requires. In this context, [n]cycloparaphenylenes ([n]CPPs) serve as excellent fluorophores for MEW processing with the additional benefit of customizable emissions profiles with the same excitation wavelength. Three fluorescent blends are used with distinct [n]CPPs with emission wavelengths of either 466, 494, or 533 nm, identifying 0.01 wt% as the preferred concentration. It is discovered that [n]CPPs disperse well within poly(ε-caprolactone) (PCL) and maintain their fluorescence even after a week of continuous heating at 80 °C. The [n]CPP-PCL blends show no cytotoxicity and support counterstaining with commonly used DAPI (Ex/Em: 359 nm/457 nm), rhodamine- (Ex/Em: 542/565 nm), and fluorescein-tagged (Ex/Em: 490/515 nm) phalloidin stains. Using different color [n]CPP-PCL blends, different MEW fibers are sequentially deposited into a semi-woven scaffold and onto a solution electrospun membrane composed of [8]CPP-PCL as a contrasting substrate for the [10]CPP-PCL MEW fibers. In general, [n]CPPs are potent fluorophores for MEW, providing new imaging options for this technology.
(© 2024 Wiley‐VCH GmbH.)
Databáze: MEDLINE
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