Complete sequencing of the Cryptosporidium suis gp60 gene reveals a novel type of tandem repeats-Implications for surveillance.

Autor: Lebbad M; Sjöbjörnsvägen, (formerly at the Department of Microbiology, Public Health Agency of Sweden, Solna, Sweden), Stockholm, Sweden., Grüttner J; European Programme for Public Health Microbiology Training (EUPHEM), European Centre for Disease Prevention and Control (ECDC), Stockholm, Sweden; Laboratory of Parasitology, Department of Bacteria, Parasites and Fungi, Statens Serum Institut, Copenhagen S, Denmark., Beser J; Department of Microbiology, Unit of Parasitology, Public Health Agency of Sweden, Solna, Sweden., Lizana V; Servicio de Análisis, Investigación y Gestión de Animales Silvestres (SAIGAS), Facultad de Veterinaria, Universidad Cardenal Herrera-CEU, CEU Universities, Valencia, Spain; Wildlife Ecology & Health Group (WE&H), Veterinary Faculty, Universitat Autònoma de Barcelona (UAB), Barcelona, Spain., Dea-Ayuela MA; Departamento de Farmacia, Facultad de Ciencias de la Salud, Universidad Cardenal Herrera-CEU, CEU Universities, Valencia, Spain., Oropeza-Moe M; Department of Production Animal Clinical Sciences, Norwegian University of Life Sciences, Sandnes, Norway., Carmena D; Parasitology Reference and Research Laboratory, Spanish National Centre for Microbiology, Health Institute Carlos III, Majadahonda, Spain; CIBER Infectious Diseases (CIBERINFEC), Health Institute Carlos III, Madrid, Spain., Stensvold CR; Laboratory of Parasitology, Department of Bacteria, Parasites and Fungi, Statens Serum Institut, Copenhagen S, Denmark. Electronic address: run@ssi.dk.
Jazyk: angličtina
Zdroj: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases [Infect Genet Evol] 2024 Aug; Vol. 122, pp. 105614. Date of Electronic Publication: 2024 Jun 04.
DOI: 10.1016/j.meegid.2024.105614
Abstrakt: Cryptosporidiosis is an infectious enteric disease caused by species (some of them zoonotic) of the genus Cryptosporidium that in many countries are under surveillance. Typing assays critical to the surveillance of cryptosporidiosis typically involve characterization of Cryptosporidium glycoprotein 60 genes (gp60). Here, we characterized the gp60 of Cryptosporidium suis from two samples-a human and a porcine faecal sample-based on which a preliminary typing scheme was developed. A conspicuous feature of the C. suis gp60 was a novel type of tandem repeats located in the 5' end of the gene and that took up 777/1635 bp (48%) of the gene. The C. suis gp60 lacked the classical poly-serine repeats (TCA/TCG/TCT), which is usually subject to major genetic variation, and the length of the tandem repeat made a typing assay incorporating this region based on Sanger sequencing practically unfeasible. We therefore designed a typing assay based on the post-repeat region only and applied it to C. suis-positive samples from suid hosts from Norway, Denmark, and Spain. We were able to distinguish three different subtypes; XXVa-1, XXVa-2, and XXVa-3. Subtype XXVa-1 had a wider geographic distribution than the other subtypes and was also observed in the human sample. We think that the present data will inform future strategies to develop a C. suis typing assay that could be even more informative by including a greater part of the gene, including the tandem repeat region, e.g., by the use of long-read next-generation sequencing.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023. Published by Elsevier B.V.)
Databáze: MEDLINE
Externí odkaz: