Current strategies using 3D organoids to establish in vitro maternal-embryonic interaction.

Autor:	Saadeldin IM; Comparative Medicine Department, King Faisal Specialist Hospital and Research Centre, Riyadh 11211, Saudi Arabia., Ehab S; Biomedical Sciences Program, University of Science and Technology, Zewail City of Science and Technology, Giza 11341, Egypt., Noreldin AE; Department of Histology and Cytology, Faculty of Veterinary Medicine, Damanhour University, the Scientific Campus, Damanhour 22511, Egypt., Swelum AA; Department of Animal Production, College of Food and Agriculture Sciences, King Saud University, Riyadh 11451, Saudi Arabia.; Department of Theriogenology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt., Bang S; College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Korea.; College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea., Kim H; Division in Biomedical Art, Department of Fine Art, Incheon Catholic University Graduate School, Incheon 21986, Korea., Yoon KY; Department of Companion Animal, Shingu College, Seongnam 13174, Korea., Lee S; College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Korea., Cho J; College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea. cjki@snu.ac.kr.
Jazyk:	angličtina
Zdroj:	Journal of veterinary science [J Vet Sci] 2024 May; Vol. 25 (3), pp. e40.
DOI:	10.4142/jvs.24004
Abstrakt:	Importance: The creation of robust maternal-embryonic interactions and implantation models is important for comprehending the early stages of embryonic development and reproductive disorders. Traditional two-dimensional (2D) cell culture systems often fail to accurately mimic the highly complex in vivo conditions. The employment of three-dimensional (3D) organoids has emerged as a promising strategy to overcome these limitations in recent years. The advancements in the field of organoid technology have opened new avenues for studying the physiology and diseases affecting female reproductive tract. Observations: This review summarizes the current strategies and advancements in the field of 3D organoids to establish maternal-embryonic interaction and implantation models for use in research and personalized medicine in assisted reproductive technology. The concepts of endometrial organoids, menstrual blood flow organoids, placental trophoblast organoids, stem cell-derived blastoids, and in vitro-generated embryo models are discussed in detail. We show the incorportaion of organoid systems and microfluidic technology to enhance tissue performance and precise management of the cellular surroundings. Conclusions and Relevance: This review provides insights into the future direction of modeling maternal-embryonic interaction research and its combination with other powerful technologies to interfere with this dialogue either by promoting or hindering it for improving fertility or methods for contraception, respectively. The merging of organoid systems with microfluidics facilitates the creation of sophisticated and functional organoid models, enhancing insights into organ development, disease mechanisms, and personalized medical investigations. Competing Interests: The authors declare no conflicts of interest. (© 2024 The Korean Society of Veterinary Science.)
Databáze:	MEDLINE
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