Detection of Mycoplasma spp. in free-living seabirds.

Autor: de Brito SG; Programa de Pós-Graduação Stricto Sensu Em Microbiologia E Parasitologia Aplicadas (PPGMPA), da Universidade Federal Fluminense, Niterói, RJ, Brazil. samaragb@id.uff.br., Dias TS; Departamento de Saúde Coletiva Veterinária E Saúde Pública, Faculdade de Veterinária, Universidade Federal Fluminense (UFF), Niterói, RJ, Brazil., Dos Santos Machado L; Departamento de Saúde Coletiva Veterinária E Saúde Pública, Faculdade de Veterinária, Universidade Federal Fluminense (UFF), Niterói, RJ, Brazil., de Souza JB; Instituto de Ciência E Tecnologia Em Biomodelos (ICTB), Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brazil., da Cunha NC; Departamento de Saúde Coletiva Veterinária E Saúde Pública, Faculdade de Veterinária, Universidade Federal Fluminense (UFF), Niterói, RJ, Brazil., de Almeida Pereira VL; Departamento de Saúde Coletiva Veterinária E Saúde Pública, Faculdade de Veterinária, Universidade Federal Fluminense (UFF), Niterói, RJ, Brazil., Barreto ML; Departamento de Imunologia (GIM), Instituto de Biologia, Universidade Federal Fluminense (UFF), Niterói, RJ, Brazil., de Mello Figueiredo Cerqueira A; Departamento de Microbiologia E Parasitologia (MIP), Instituto Biomédico (CMB), Universidade Federal Fluminense (UFF), Niterói, RJ, Brazil., Neves FPG; Programa de Pós-Graduação Stricto Sensu Em Microbiologia E Parasitologia Aplicadas (PPGMPA), da Universidade Federal Fluminense, Niterói, RJ, Brazil.; Departamento de Microbiologia E Parasitologia (MIP), Instituto Biomédico (CMB), Universidade Federal Fluminense (UFF), Niterói, RJ, Brazil.
Jazyk: angličtina
Zdroj: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2024 Sep; Vol. 55 (3), pp. 2937-2942. Date of Electronic Publication: 2024 Jun 04.
DOI: 10.1007/s42770-024-01409-8
Abstrakt: This study aimed to investigate the presence of Mycoplasma spp. and identify the species of mycoplasma isolates obtained from seabirds found on Brazilian coastal beaches. Tracheal and cloacal swab samples were collected from 50 seabirds rescued by three conservation and marine animal rehabilitation centers located in Brazil. The tracheal and cloacal samples were subjected to mycoplasma culture and the isolates were identified through PCR. A "Mollicutes-specific" 16S rRNA PCR reaction was employed for triage. Four species-specific PCR reactions were used to detect Mycoplasma gallisepticum, Mycoplasma synoviae, Mycoplasma meleagridis, or M. gallinarum. The Mollicutes positive and species negative samples were submitted do 16S rRNA sequencing. Eighteen (36%) of 50 seabirds tested positive for mycoplasma by culture. In the PCR for the genus, 28 (56%) of 50 seabirds were positive for Mycoplasma spp., with 13 (26%) detected in the trachea, one (2%) in the cloaca, and 14 (28%) in both sites. In the species-specific PCR, M. gallisepticum was detected in 17.8%, and M. meleagridis in 17.8%. Both species were detected in 14.3%. Of the isolates not characterized at species level, we obtained ten sequences and they were divided into three clusters. The first cluster was closely related to M. meleagridis, the second to M. synoviae, and the third grouped M. tully, M. gallisepticum, and M. imitans. Four and five of nine species of seabirds studied had mycoplasma detected by culture or PCR, respectively. Mycoplasmas were found in the majority of the animals studied, with the highest prevalence proportionally found in Sula leucogaster, and the lowest in Fregata magnificens. The phylogenetic analysis identified Mycoplasma spp. adapted to aquatic birds.
(© 2024. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.)
Databáze: MEDLINE
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