| Autor: |
Tennant GB, Bailey-Wood R, Dallimore CM |
| Jazyk: |
angličtina |
| Zdroj: |
Cell and tissue kinetics [Cell Tissue Kinet] 1985 Mar; Vol. 18 (2), pp. 147-53. |
| DOI: |
10.1111/j.1365-2184.1985.tb00643.x |
| Abstrakt: |
The effect of medium osmolality was examined in primary, continuous bone-marrow cultures established from TO strain mice. The non-adherent cell population increased exponentially between weeks 2 and 5 and thereafter declined steadily. The number of CFU-GM followed a similar pattern but showed greater variability. The optimum osmolality in 4 week old cultures was found to be about 345 mosmol/kg which was higher than the plasma osmolality (n = 20; mean = 323.3 mosmol/kg; range = 313-331). Maximum non-adherent cell numbers were found at about 345 mosmol/kg (better than half-maximum between 320 and 370 mosmol/kg). CFU-GM numbers in the culture supernatant were maximal at about 355 mosmol/kg (better than half-maximum between 320 and 400 mosmol/kg). An adherent layer developed over a wider range of osmolality than supported granulopoiesis (better than half-maximum between 258 and 402 mosmol/kg). It was necessary to increase the osmolality of Fischer's medium in order to obtain maximum growth. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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