Visualization of Bacillus subtilis spore structure and germination using quick-freeze deep-etch electron microscopy.

Autor:	Jalil K; Graduate School of Science, Osaka Metropolitan University, Sumiyoshi-ku, Osaka 558-8585, Japan., Tahara YO; Graduate School of Science, Osaka Metropolitan University, Sumiyoshi-ku, Osaka 558-8585, Japan.; The OCU Advanced Research Institute for Natural Science and Technology (OCARINA), Osaka Metropolitan University, Sumiyoshi-ku, Osaka 558-8585, Japan., Miyata M; Graduate School of Science, Osaka Metropolitan University, Sumiyoshi-ku, Osaka 558-8585, Japan.; The OCU Advanced Research Institute for Natural Science and Technology (OCARINA), Osaka Metropolitan University, Sumiyoshi-ku, Osaka 558-8585, Japan.
Jazyk:	angličtina
Zdroj:	Microscopy (Oxford, England) [Microscopy (Oxf)] 2024 Dec 05; Vol. 73 (6), pp. 463-472.
DOI:	10.1093/jmicro/dfae023
Abstrakt:	Bacterial spores, known for their complex and resilient structures, have been the focus of visualization using various methodologies. In this study, we applied quick-freeze and replica electron microscopy techniques, allowing observation of Bacillus subtilis spores in high-contrast and three-dimensional detail. This method facilitated visualization of the spore structure with enhanced resolution and provided new insights into the spores and their germination processes. We identified and described five distinct structures: (i) hair-like structures on the spore surface, (ii) spike formation on the surface of lysozyme-treated spores, (iii) the fractured appearance of the spore cortex during germination, (iv) potential connections between small vesicles and the core membrane and (v) the evolving surface structure of nascent vegetative cells during germination. (© The Author(s) 2024. Published by Oxford University Press on behalf of The Japanese Society of Microscopy.)
Databáze:	MEDLINE
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