MeGT2.6 increases cellulose synthesis and active gibberellin content to promote cell enlargement in cassava.

Autor: Bao R; Sanya Institute of Breeding and Multiplication, Hainan University/National Key Laboratory for Tropical Crop Breeding, Sanya, 572025, Hainan, China., Zeng C; Sanya Institute of Breeding and Multiplication, Hainan University/National Key Laboratory for Tropical Crop Breeding, Sanya, 572025, Hainan, China., Li K; Sanya Institute of Breeding and Multiplication, Hainan University/National Key Laboratory for Tropical Crop Breeding, Sanya, 572025, Hainan, China., Li M; Sanya Institute of Breeding and Multiplication, Hainan University/National Key Laboratory for Tropical Crop Breeding, Sanya, 572025, Hainan, China., Li Y; Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, Hainan, China.; Key Laboratory for Biology and Genetic Resources of Tropical Crops of Hainan Province, Hainan Institute for Tropical Agricultural Resources, Haikou, 571101, Hainan, China., Zhou X; Sanya Institute of Breeding and Multiplication, Hainan University/National Key Laboratory for Tropical Crop Breeding, Sanya, 572025, Hainan, China.; Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, Hainan, China.; Key Laboratory for Biology and Genetic Resources of Tropical Crops of Hainan Province, Hainan Institute for Tropical Agricultural Resources, Haikou, 571101, Hainan, China.; Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Sanya, 572025, Hainan, China., Wang H; Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, Hainan, China.; Key Laboratory for Biology and Genetic Resources of Tropical Crops of Hainan Province, Hainan Institute for Tropical Agricultural Resources, Haikou, 571101, Hainan, China., Wang Y; Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, Hainan, China.; Key Laboratory for Biology and Genetic Resources of Tropical Crops of Hainan Province, Hainan Institute for Tropical Agricultural Resources, Haikou, 571101, Hainan, China.; Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Sanya, 572025, Hainan, China., Huang D; Sanya Institute of Breeding and Multiplication, Hainan University/National Key Laboratory for Tropical Crop Breeding, Sanya, 572025, Hainan, China., Wang W; Sanya Institute of Breeding and Multiplication, Hainan University/National Key Laboratory for Tropical Crop Breeding, Sanya, 572025, Hainan, China., Chen X; Sanya Institute of Breeding and Multiplication, Hainan University/National Key Laboratory for Tropical Crop Breeding, Sanya, 572025, Hainan, China.; Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, Hainan, China.; Key Laboratory for Biology and Genetic Resources of Tropical Crops of Hainan Province, Hainan Institute for Tropical Agricultural Resources, Haikou, 571101, Hainan, China.; Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Sanya, 572025, Hainan, China.
Jazyk: angličtina
Zdroj: The Plant journal : for cell and molecular biology [Plant J] 2024 Jul; Vol. 119 (2), pp. 1014-1029. Date of Electronic Publication: 2024 May 28.
DOI: 10.1111/tpj.16813
Abstrakt: Cassava, a pivotal tropical crop, exhibits rapid growth and possesses a substantial biomass. Its stem is rich in cellulose and serves as a crucial carbohydrate storage organ. The height and strength of stems restrict the mechanised operation and propagation of cassava. In this study, the triple helix transcription factor MeGT2.6 was identified through yeast one-hybrid assay using MeCesA1pro as bait, which is critical for cellulose synthesis. Over-expression and loss-of-function lines were generated, and results revealed that MeGT2.6 could promote a significant increase in the plant height, stem diameter, cell size and thickness of SCW of cassava plant. Specifically, MeGT2.6 upregulated the transcription activity of MeGA20ox1 and downregulated the expression level of MeGA2ox1, thereby enhancing the content of active GA3, resulting in a large cell size, high plant height and long stem diameter in cassava. Moreover, MeGT2.6 upregulated the transcription activity of MeCesA1, which promoted the synthesis of cellulose and hemicellulose and produced a thick secondary cell wall. Finally, MeGT2.6 could help supply additional substrates for the synthesis of cellulose and hemicellulose by upregulating the invertase genes (MeNINV1/6). Thus, MeGT2.6 was found to be a multiple regulator; it was involved in GA metabolism and sucrose decomposition and the synthesis of cellulose and hemicellulose.
(© 2024 Society for Experimental Biology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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