RNA interference as a next-generation control method for suppressing Varroa destructor reproduction in honey bee (Apis mellifera) hives.

Autor: McGruddy RA; School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand., Smeele ZE; School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand., Manley B; GreenLight Biosciences, Research Triangle Park, Durham, NC, USA., Masucci JD; GreenLight Biosciences, Research Triangle Park, Durham, NC, USA., Haywood J; School of Mathematics and Statistics, Victoria University of Wellington, Wellington, New Zealand., Lester PJ; School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand.
Jazyk: angličtina
Zdroj: Pest management science [Pest Manag Sci] 2024 Sep; Vol. 80 (9), pp. 4770-4778. Date of Electronic Publication: 2024 May 27.
DOI: 10.1002/ps.8193
Abstrakt: Background: The Varroa mite (Varroa destructor) is considered to be the greatest threat to apiculture worldwide. RNA interference (RNAi) using double-stranded RNA (dsRNA) as a gene silencing mechanism has emerged as a next-generation strategy for mite control.
Results: We explored the impact of a dsRNA biopesticide, named vadescana, designed to silence the calmodulin gene in Varroa, on mite fitness in mini-hives housed in a laboratory. Two dosages were tested: 2 g/L dsRNA and 8 g/L dsRNA. Vadescana appeared to have no effect on mite survival, however, mite fertility was substantially reduced. The majority of foundress mites exposed to vadescana failed to produce any offspring. No dose-dependent effect of vadescana was observed, as both the low and high doses inhibited mite reproduction equally well in the mini-hives and neither dose impacted pupal survival of the honey bee. Approximately 95% of bee pupae were alive at uncapping across all treatment groups.
Conclusion: These findings suggest that vadescana has significant potential as an effective alternative to conventional methods for Varroa control, with broader implications for the utilization of RNAi as a next-generation tool in the management of pest species. © 2024 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
(© 2024 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.)
Databáze: MEDLINE
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