A biocompatible surface display approach in Shewanella promotes current output efficiency.

Autor: Zhao J; State Key Laboratory of Coordination Chemistry, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Life Sciences, Nanjing University, Nanjing, 210023, China., Wang C; School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, China., Liu J; State Key Laboratory of Coordination Chemistry, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Life Sciences, Nanjing University, Nanjing, 210023, China., Zhang N; State Key Laboratory of Coordination Chemistry, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Life Sciences, Nanjing University, Nanjing, 210023, China., Zhao Y; State Key Laboratory of Coordination Chemistry, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Life Sciences, Nanjing University, Nanjing, 210023, China., Zhao J; State Key Laboratory of Coordination Chemistry, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Life Sciences, Nanjing University, Nanjing, 210023, China; School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, China; NJU Xishan Institute of Applied Biotechnology, Wuxi, 214000, China. Electronic address: jingzhao@nju.edu.cn., Wang X; State Key Laboratory of Coordination Chemistry, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Life Sciences, Nanjing University, Nanjing, 210023, China; School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, China; NJU Xishan Institute of Applied Biotechnology, Wuxi, 214000, China. Electronic address: wangxiuxiu@nju.edu.cn., Wei W; State Key Laboratory of Coordination Chemistry, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Life Sciences, Nanjing University, Nanjing, 210023, China; NJU Xishan Institute of Applied Biotechnology, Wuxi, 214000, China. Electronic address: weiwei@nju.edu.cn.
Jazyk: angličtina
Zdroj: Biosensors & bioelectronics [Biosens Bioelectron] 2024 Sep 01; Vol. 259, pp. 116422. Date of Electronic Publication: 2024 May 23.
DOI: 10.1016/j.bios.2024.116422
Abstrakt: The biology-material hybrid method for chemical-electricity conversion via microbial fuel cells (MFCs) has garnered significant attention in addressing global energy and environmental challenges. However, the efficiency of these systems remains unsatisfactory due to the complex manufacturing process and limited biocompatibility. To overcome these challenges, here, we developed a simple bio-inorganic hybrid system for bioelectricity generation in Shewanella oneidensis (S. oneidensis) MR-1. A biocompatible surface display approach was designed, and silver-binding peptide AgBP2 was expressed on the cell surface. Notably, the engineered Shewanella showed a higher electrochemical sensitivity to Ag + , and a 60 % increase in power density was achieved even at a low concentration of 10 μM Ag + . Further analysis revealed significant upregulations of cell surface negative charge intensity, ATP metabolism, and reducing equivalent (NADH/NAD + ) ratio in the engineered S. oneidensis-Ag nanoparticles biohybrid. This work not only provides a novel insight for electrochemical biosensors to detect metal ions, but also offers an alternative biocompatible surface display approach by combining compatible biomaterials with electricity-converting bacteria for advancements in biohybrid MFCs.
Competing Interests: Declaration of competing interest The authors declare no competing financial interest.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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