| Autor: |
Chen YM; Department of Microbiology and Immunology, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan.; Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan.; Molecular Infectious Disease Research Center, Chang Gung Memorial Hospital, Linkou 333, Taiwan., Yang YC; Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan., Shieh HR; Department of Microbiology and Immunology, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan., Lin YJ; Department of Microbiology and Immunology, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan., Ke WJ; Department of Microbiology and Immunology, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan., Chiu CH; Molecular Infectious Disease Research Center, Chang Gung Memorial Hospital, Linkou 333, Taiwan. |
| Abstrakt: |
The pil gene cluster for Type IV pilus (Tfp) biosynthesis is commonly present and highly conserved in Streptococcus sanguinis . Nevertheless, Tfp-mediated twitching motility is less common among strains, and the factors determining twitching activity are not fully understood. Here, we analyzed the functions of three major pilin proteins (PilA1, PilA2, and PilA3) in the assembly and activity of Tfp in motile S. sanguinis CGMH010. Using various recombinant pilA deletion strains, we found that Tfp composed of different PilA proteins varied morphologically and functionally. Among the three PilA proteins, PilA1 was most critical in the assembly of twitching-active Tfp, and recombinant strains expressing motility generated more structured biofilms under constant shearing forces compared to the non-motile recombinant strains. Although PilA1 and PilA3 shared 94% identity, PilA3 could not compensate for the loss of PilA1, suggesting that the nature of PilA proteins plays an essential role in twitching activity. The single deletion of individual pilA genes had little effect on the invasion of host endothelia by S. sanguinis CGMH010. In contrast, the deletion of all three pilA genes or pilT , encoding the retraction ATPase, abolished Tfp-mediated invasion. Tfp- and PilT-dependent invasion were also detected in the non-motile S. sanguinis SK36, and thus, the retraction of Tfp, but not active twitching, was found to be essential for invasion. |
