Shifts in uterine microbiome associated with pregnancy outcomes at first insemination and clinical cure in dairy cows with metritis.

Autor: Figueiredo CC; Department of Large Animal Clinical Sciences, D. H. Barron Reproductive and Perinatal Biology Research Program, University of Florida, Gainesville, 32610, USA.; Department of Veterinary Clinical Sciences, Washington State University, Pullman, 99164, USA., Monteiro HF; Department of Population Health and Reproduction, University of California, Davis, 95616, USA., Cunha F; Department of Large Animal Clinical Sciences, D. H. Barron Reproductive and Perinatal Biology Research Program, University of Florida, Gainesville, 32610, USA., Bisinotto DZ; Department of Large Animal Clinical Sciences, D. H. Barron Reproductive and Perinatal Biology Research Program, University of Florida, Gainesville, 32610, USA., Ruiz AR; Department of Animal Sciences, University of Florida, Gainesville, 32611, USA., Duarte GA; Department of Animal Sciences, University of Florida, Gainesville, 32611, USA., Ge Y; Department of Microbiology, Immunology and Molecular Genetics, University of Texas Health, San Antonio, 78229, USA., Lima FS; Department of Population Health and Reproduction, University of California, Davis, 95616, USA., Mohamadzadeh M; Department of Microbiology, Immunology and Molecular Genetics, University of Texas Health, San Antonio, 78229, USA., Galvão KN; Department of Large Animal Clinical Sciences, D. H. Barron Reproductive and Perinatal Biology Research Program, University of Florida, Gainesville, 32610, USA., Bisinotto RS; Department of Large Animal Clinical Sciences, D. H. Barron Reproductive and Perinatal Biology Research Program, University of Florida, Gainesville, 32610, USA. rsbisinotto@ufl.edu.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 May 24; Vol. 14 (1), pp. 11864. Date of Electronic Publication: 2024 May 24.
DOI: 10.1038/s41598-024-61704-0
Abstrakt: Objectives were to assess differences in uterine microbiome associated with clinical cure and pregnancy outcomes in dairy cows treated for metritis. Cows with metritis (reddish-brownish, watery, and fetid vaginal discharge) were paired with cows without metritis based on parity and days postpartum. Uterine contents were collected through transcervical lavage at diagnosis, five days later following antimicrobial therapy (day 5), and at 40 days postpartum. Uterine microbiome was assessed by sequencing the V4 hypervariable region of the 16S rRNA gene. Although alpha-diversity based on Chao1, Shannon, and inverse Simpson indexes at diagnosis did not differ between cows with and without metritis, disease was associated with differences in beta-diversity. Prevalence of Porphyromonas, Bacteroides, and Veillonella was greater in cows with metritis. Streptococcus, Sphingomonas, and Ureaplasma were more prevalent in cows without metritis. Differences in beta-diversity between cows with and without metritis persisted on day 5. Uterine microbiome was not associated with clinical cure. Richness and alpha-diversity, but not beta-diversity, of uterine microbiome 40 days postpartum were associated with metritis and pregnancy. No relationship between uterine microbiome and pregnancy outcomes was observed. Results indicate that factors other than changes in intrauterine bacterial community underlie fertility loss and clinical cure in cows with metritis.
(© 2024. The Author(s).)
Databáze: MEDLINE
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