Acute toxicity appraisal of Saussurea heteromalla extract and development of its chemotherapeutic herbal dosage form.

Autor: Batool A; Department of Oncology, The First Affiliated Hospital of Xinxiang Medical University Weihui, Henan Province, P.R. China.; Department of Pharmaceutical Chemistry, Hamdard Institute of Pharmaceutical Sciences, Hamdard University, Islamabad, Pakistan., Muddassir M; Department of Quality Control, Amson vaccines and Pharma, Islamabad, Pakistan., Alshammari BH; Faculty of Science, Chemistry Department, University of Hail, Hail, Saudi Arabia., Alsahli TG; Department of Pharmacology, College of Pharmacy, Jouf University, Sakaka, Aljouf, Saudi Arabia., Solre GFB; Tianjin Key Laboratory for Modern Drug Delivery and High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, P.R. China., Liu Y; Department of Oncology, The First Affiliated Hospital of Xinxiang Medical University Weihui, Henan Province, P.R. China., Khan MT; Faculty of Pharmacy, Capital University of Science and Technology, Islamabad, Pakistan., Malik NS; Faculty of Pharmacy, Capital University of Science and Technology, Islamabad, Pakistan., Ahsan H; Department of Pharmaceutics, Riphah Institute of Pharmaceutical Sciences, Riphah International University, Islamabad, Pakistan., Khan MT; Zhongjing Research and Industrialization Institute of Chinese Medicine, Zhongguancun Scientific Park, Meixi, Nanyang, Henan, P.R. China.; Institute of Molecular Biology and Biotechnology (IMBB), The University of Lahore, Lahore, Pakistan., Malik MNH; Faculty of Pharmacy, The University of Lahore, Lahore, Pakistan., Miana GA; Department of Pharmaceutical Chemistry, Hamdard Institute of Pharmaceutical Sciences, Hamdard University, Islamabad, Pakistan.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2024 May 24; Vol. 19 (5), pp. e0302657. Date of Electronic Publication: 2024 May 24 (Print Publication: 2024).
DOI: 10.1371/journal.pone.0302657
Abstrakt: Ethnopharmacological relevance of Saussurea species for anti-cancer compounds instigated us to develop chemotherapeutic herbal tablets. This study was an ongoing part of our previous research based on the scientific evaluation of Saussurea heteromalla (S. heteromalla) for anti-cancer lead compounds. In the current study, S. heteromalla herbal tablets (500 /800 mg) were designed and evaluated for anti-cancer activity. Arctigenin was found as a bioactive lead molecule with anti-cancer potential for cervical cancer. The in vitro results on the HeLa cell line supported the ethnopharmacological relevance and traditional utilization of S. heteromalla and provided the scientific basis for the management of cervical cancer as proclaimed by traditional practitioners in China. LD50 of the crude extract was established trough oral acute toxicity profiling in mice, wherein the minimum lethal dose was noticed as higher than 1000 mg/kg body weight orally. Chromatographic fingerprint analysis ensured the identity and consistency of S. heteromalla in herbal tablets in terms of standardization of the herbal drug. About 99.15% of the drug (S. heteromalla crude extract) was recovered in herbal tablets (RSD: 0.45%). In vitro drug release profile was found to be more than 87% within 1 h, which was also correlated with different mathematical kinetic models of drug release (r2 = 0.992), indicating that drug release from matrix tablets into the blood is constant throughout the delivery. The dosage form was found stable after an accelerated stability parameters study which may be used for anti-cervical cancer therapy in the future, if it qualifies successful preclinical investigation parameters.
Competing Interests: All authors declare that they do not have a conflict of interest relating to conduct of the study or publication of this manuscript.
(Copyright: © 2024 Batool et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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