| Autor: |
Arinbasarova AY; G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290 Pushchino, Russia., Botin AS; Institute of Biochemical Technology and Nanotechnology (IBTN), Peoples' Friendship University of Russia na. P.Lumumba (RUDN), 6 Miklukho-Maklaya St., 117198 Moscow, Russia.; N.V. Sklifosovsky Institute of Emergency Medicine, 129090 Moscow, Russia., Medentsev AG; G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290 Pushchino, Russia., Makrushin KV; G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290 Pushchino, Russia., Vetcher AA; Institute of Biochemical Technology and Nanotechnology (IBTN), Peoples' Friendship University of Russia na. P.Lumumba (RUDN), 6 Miklukho-Maklaya St., 117198 Moscow, Russia., Stanishevskiy YM; Institute of Biochemical Technology and Nanotechnology (IBTN), Peoples' Friendship University of Russia na. P.Lumumba (RUDN), 6 Miklukho-Maklaya St., 117198 Moscow, Russia. |
| Abstrakt: |
When cultivating on wheat bran or deactivated fungal mycelium as a model of "natural growth", the ability of Trichoderma to synthesize extracellular L-lysine-α-oxidase (LysO) simultaneously with cell-wall-degrading enzymes (proteases, xylanase, glucanases, chitinases, etc.), responsible for mycoparasitism, was shown. LysO, in turn, causes the formation of H 2 O 2 and pipecolic acid. These compounds are known to be signaling molecules and play an important role in the induction and development of systemic acquired resistance in plants. Antagonistic effects of LysO have been demonstrated against phytopathogenic fungi and Gram-positive or Gram-negative bacteria with dose-dependent cell death. The antimicrobial effect of LysO decreased in the presence of catalase. The generating intracellular ROS in the presence of LysO was also shown in both bacteria and fungi, which led to a decrease in viable cells. These results suggest that the antimicrobial activity of LysO is due to two factors: the formation of exogenous hydrogen peroxide as a product of the enzymatic oxidative deamination of L-lysine and the direct interaction of LysO with the cell wall of the micro-organisms. Thus, LysO on its own enhances the potential of the producer in the environment; namely, the enzyme complements the strategy of the fungus in biocontrol and indirectly participates in inducing SAR and regulating the relationship between pathogens and plants. |
