| Autor: |
Nakatani N; Department of Pediatrics, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan., Win KHN; Department of Public Health, Kobe University Graduate School of Health Science, Kobe 654-0142, Japan., Mon CY; Department of Public Health, Kobe University Graduate School of Health Science, Kobe 654-0142, Japan., Fujikawa T; Department of Pediatrics, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan., Uemura S; Department of Hematology/Oncology, Kobe Children's Hospital, Kobe 650-0047, Japan., Saito A; Department of Hematology/Oncology, Kobe Children's Hospital, Kobe 650-0047, Japan., Ishida T; Department of Hematology/Oncology, Kobe Children's Hospital, Kobe 650-0047, Japan., Mori T; Department of Hematology/Oncology, Kobe Children's Hospital, Kobe 650-0047, Japan., Hasegawa D; Department of Hematology/Oncology, Kobe Children's Hospital, Kobe 650-0047, Japan., Kosaka Y; Department of Hematology/Oncology, Kobe Children's Hospital, Kobe 650-0047, Japan., Inoue S; Department of Pediatrics, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan., Nishimura A; Department of Pediatrics, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan., Nino N; Department of Hematology/Oncology, Kobe Children's Hospital, Kobe 650-0047, Japan., Tamura A; Department of Pediatrics, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan., Yamamoto N; Department of Pediatrics, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan., Nozu K; Department of Pediatrics, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan., Nishimura N; Department of Public Health, Kobe University Graduate School of Health Science, Kobe 654-0142, Japan. |
| Abstrakt: |
Non-high-risk (non-HR) neuroblastoma (NB) patients have excellent outcomes, with more than a 90% survival rate, whereas HR NB patients expect less than a 50% survival rate. Metastatic disease is the principal cause of death among both non-HR and HR NB patients. Previous studies have reported the significant but limited prognostic value of quantitative PCR (qPCR)-based assays, measuring overlapping but different sets of neuroblastoma-associated mRNAs (NB-mRNAs), to detect metastatic disease in both non-HR and HR patient samples. A droplet digital PCR (ddPCR)-based assay measuring seven NB-mRNAs (CRMP1, DBH, DDC, GAP43, ISL1, PHOX2B, and TH mRNAs) was recently developed and exhibited a better prognostic value for HR patient samples than qPCR-based assays. However, it remained to be tested on non-HR patient samples. In the present study, we employed the ddPCR-based assay to study peripheral blood (PB) and bone marrow (BM) samples collected at diagnosis from eight non-HR and eleven HR cases and characterized the expression profiles of NB-mRNAs. The most highly expressed NB-mRNAs in PB and BM differed between non-HR and HR cases, with the CRMP1 mRNA being predominant in non-HR cases and the GAP43 mRNA in HR cases. The levels of NB-mRNAs in PB and BM were 5 to 1000 times lower in non-HR cases than in HR cases. The PB to BM ratio of NB-mRNAs was 10 to 100 times higher in non-HR cases compared to HR cases. The present case series suggests that non-HR and HR NB patients have the distinct expression profiles of NB-mRNAs in their PB and BM. |
