Heat-killed Akkermansia muciniphila ameliorates allergic airway inflammation in mice.

Autor:	Yoon SA; R&D Center, Enterobiome Inc., Ilsandong-gu, Goyang-si, Republic of Korea., Lim Y; R&D Center, Enterobiome Inc., Ilsandong-gu, Goyang-si, Republic of Korea., Byeon HR; R&D Center, Enterobiome Inc., Ilsandong-gu, Goyang-si, Republic of Korea., Jung J; R&D Center, Enterobiome Inc., Ilsandong-gu, Goyang-si, Republic of Korea., Ma S; R&D Center, Enterobiome Inc., Ilsandong-gu, Goyang-si, Republic of Korea., Hong MG; R&D Center, Enterobiome Inc., Ilsandong-gu, Goyang-si, Republic of Korea., Kim D; R&D Center, Enterobiome Inc., Ilsandong-gu, Goyang-si, Republic of Korea., Song EJ; Research Group of Personalized Diet, Korea Food Research Institute, Wanju-gun, Jeollabuk-do, Republic of Korea., Nam YD; Research Group of Personalized Diet, Korea Food Research Institute, Wanju-gun, Jeollabuk-do, Republic of Korea., Seo JG; R&D Center, Enterobiome Inc., Ilsandong-gu, Goyang-si, Republic of Korea., Lee SN; R&D Center, Enterobiome Inc., Ilsandong-gu, Goyang-si, Republic of Korea.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2024 May 09; Vol. 15, pp. 1386428. Date of Electronic Publication: 2024 May 09 (Print Publication: 2024).
DOI:	10.3389/fmicb.2024.1386428
Abstrakt:	Allergic asthma (AA) is a common inflammatory airway disease characterized by increased airway hyper-responsiveness (AHR), inflammation, and remodeling. Akkermansia muciniphila is a strictly anaerobic bacterium residing in the gut and is a promising next-generation probiotic to improve metabolic inflammatory syndrome. A recent study suggested the beneficial effect of live A. muciniphila on allergic airway inflammation (AAI) in mice. However, whether the heat-killed form can improve AAI requires further investigation. Mice sensitized and challenged with house dust mites (HDM) develop AA hallmarks including inflammatory cell infiltration, goblet cell hyperplasia, and subepithelial collagen deposition in the lungs. These phenomena were reversed by oral administration of the heat-killed A. muciniphila strain EB-AMDK19 (AMDK19-HK) isolated from the feces of healthy Koreans. Furthermore, AMDK19-HK diminished the HDM-induced AHR to inhaled methacholine, lung mast cell accumulation, and serum HDM-specific IgE levels. It also led to the overall suppression of IL-4, IL-13, and eotaxin production in bronchoalveolar lavage fluids, and Il4 , Il5 , Il13 , and Ccl17 gene expression in lung tissues. Moreover, AMDK19-HK suppressed Th2-associated cytokine production in the splenocytes of HDM-sensitized mice in vitro . Additionally, a combination of 16S rRNA gene sequencing and short-chain fatty acid (SCFA) analysis in cecal samples revealed that AMDK19-HK modulated the relative abundance of circulating SCFA-associated gut genera, including a positive correlation with Lachnospiraceae_ NK4A136_group and a negative correlation with Lachnoclostridium and significantly increased cecal SCFA concentrations. Finally, AMDK19-HK improved intestinal mucosal barrier function. These results suggest that the oral administration of AMDK19-HK ameliorates HDM-induced AAI in mice by suppressing Th2-mediated immune responses and could have a protective effect against AA development. Competing Interests: SY, YL, HB, JJ, SM, M-GH, DK, J-GS, and S-NL are full-time employees of Enterobiome Inc. SY, YL, HB, JJ, SM, M-GH, DK, and J-GS are stock/stock option shareholders of Enterobiome Inc. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Yoon, Lim, Byeon, Jung, Ma, Hong, Kim, Song, Nam, Seo and Lee.)
Databáze:	MEDLINE
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