Antifungal activity of aminopyrrolnitrin against Trichophyton verrucosum in a guinea pig model of dermatophytosis.

Autor: Lee HG; Division of Animal Diseases & Health, National Institute of Animal Science, Rural Development Administration, Wanju, Republic of Korea.; Department of Veterinary Infectious Disease, College of Veterinary Medicine, Jeonbuk National University, Iksan, Republic of Korea., Bok EY; Division of Animal Diseases & Health, National Institute of Animal Science, Rural Development Administration, Wanju, Republic of Korea., Jung YH; Division of Animal Diseases & Health, National Institute of Animal Science, Rural Development Administration, Wanju, Republic of Korea., Hur TY; Division of Animal Diseases & Health, National Institute of Animal Science, Rural Development Administration, Wanju, Republic of Korea., Kim YO; Biotechnology Research Division, National Institute of Fisheries Science, Busan, Republic of Korea., Kong HJ; Biotechnology Research Division, National Institute of Fisheries Science, Busan, Republic of Korea., Kim DG; Biotechnology Research Division, National Institute of Fisheries Science, Busan, Republic of Korea., Kim YS; Biotechnology Research Division, National Institute of Fisheries Science, Busan, Republic of Korea., Oem JK; Department of Veterinary Infectious Disease, College of Veterinary Medicine, Jeonbuk National University, Iksan, Republic of Korea.
Jazyk: angličtina
Zdroj: Mycoses [Mycoses] 2024 May; Vol. 67 (5), pp. e13748.
DOI: 10.1111/myc.13748
Abstrakt: Background: Dermatophytosis is a common and major public health concern worldwide. Despite the increasing availability of antifungal drugs, relapses and untreated cases of dermatophyte infections are reported. Therefore, novel antifungal agents are required. Aminopyrrolnitrin (APRN) shows promise for dermatophytosis treatment because of its antifungal activity.
Objectives: This study aimed to assess the antifungal properties of APRN against Trichophyton verrucosum (T. verrucosum), in both laboratory settings and a guinea pig model.
Methods: The minimum inhibitory concentrations (MICs) of APRN and enilconazole against T. verrucosum were determined according to the CLSI M38 method. The skins of 16 male guinea pigs were infected with 1.0 × 10 8 conidia of T. verrucosum and the animals were grouped into sets of four: negative control group (NC) received normal saline; positive control group (PC) received 2 μg/mL of enilconazole; and APRN4 and APRN8 received 4 and 8 μg/mL of APRN, respectively. Clinical, mycological and histological efficacies were measured after 10 days.
Results: The MIC 90 of APRN and enilconazole against T. verrucosum was 4 and 2 μg/mL, respectively. The clinical scores of PC, APRN4, and APRN8 were significantly lower than those of NC. Clinical and mycological efficacies were higher for APRN8, APRN4 and PC. No fungi were observed in the skin tissues of APRN4 and APRN8, while fungi were observed in 50% of the PC.
Conclusion: APRN showed antifungal activity against T. verrucosum in vitro and in vivo and is a potential candidate for the treatment of dermatophytosis.
(© 2024 The Author(s). Mycoses published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
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