Development of pH-responsive Eudragit S100-functionalized silk fibroin nanoparticles as a prospective drug delivery system.

Autor: Pham DT; Department of Chemistry, College of Natural Sciences, Can Tho University, Can Tho, Vietnam., Nguyen DXT; Department of Chemistry, College of Natural Sciences, Can Tho University, Can Tho, Vietnam., Nguyen NY; Department of Chemistry, College of Natural Sciences, Can Tho University, Can Tho, Vietnam., Nguyen TTL; Department of Chemistry, College of Natural Sciences, Can Tho University, Can Tho, Vietnam., Nguyen TQC; Department of Chemistry, College of Natural Sciences, Can Tho University, Can Tho, Vietnam., Tu AVT; Department of Biology, College of Natural Sciences, Can Tho University, Can Tho, Vietnam., Nguyen NH; Department of Biostatistics and Demography, Faculty of Public Health, Can Tho University of Medicine and Pharmacy, Can Tho, Vietnam., Thuy BTP; Faculty of Fundamental Sciences, Van Lang University, Ho Chi Minh City, Vietnam.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2024 May 23; Vol. 19 (5), pp. e0303177. Date of Electronic Publication: 2024 May 23 (Print Publication: 2024).
DOI: 10.1371/journal.pone.0303177
Abstrakt: Silk fibroin nanoparticles (FNP) have been increasingly investigated in biomedical fields due to their biocompatibility and biodegradability properties. To widen the FNP versatility and applications, and to control the drug release from the FNP, this study developed the Eudragit S100-functionalized FNP (ES100-FNP) as a pH-responsive drug delivery system, by two distinct methods of co-condensation and adsorption, employing the zwitterionic furosemide as a model drug. The particles were characterized by sizes and zeta potentials (DLS method), morphology (electron microscopy), drug entrapment efficiency and release profiles (UV-Vis spectroscopy), and chemical structures (FT-IR, XRD, and DSC). The ES100-FNP possessed nano-sizes of ∼200-350 nm, zeta potentials of ∼ -20 mV, silk-II structures, enhanced thermo-stability, non-cytotoxic to the erythrocytes, and drug entrapment efficiencies of 30%-60%, dependent on the formulation processes. Interestingly, the co-condensation method yielded the smooth spherical particles, whereas the adsorption method resulted in durian-shaped ones due to furosemide re-crystallization. The ES100-FNP adsorbed furosemide via physical adsorption, followed Langmuir model and pseudo-second-order kinetics. In the simulated oral condition, the particles could protect the drug in the stomach (pH 1.2), and gradually released the drug in the intestine (pH 6.8). Remarkably, in different pH conditions of 6.8, 9.5, and 12, the ES100-FNP could control the furosemide release rates depending on the formulation methods. The ES100-FNP made by the co-condensation method was mainly controlled by the swelling and corrosion process of ES100, and followed the Korsmeyer-Peppas non-Fickian transport mechanism. Whereas, the ES100-FNP made by the adsorption method showed constant release rates, followed the zero-order kinetics, due to the gradual furosemide dissolution in the media. Conclusively, the ES100-FNP demonstrated high versatility as a pH-responsive drug delivery system for biomedical applications.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Pham et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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