Effects of plant protease inhibitors (Pep-3-EcTI, Pep-BbKI, and Pep-BrTI) versus corticosteroids on inflammation, remodeling, and oxidative stress in an asthma-COPD (ACO) model.

Autor:	João JMLG; Faculdade de Medicina FMUSP, Universidade de São Paulo, São Paulo, Brazil., Silva Barbosa JA; Faculdade de Medicina FMUSP, Universidade de São Paulo, São Paulo, Brazil., Sales da Silva LL; Faculdade de Medicina FMUSP, Universidade de São Paulo, São Paulo, Brazil., Fukuzaki S; Faculdade de Medicina FMUSP, Universidade de São Paulo, São Paulo, Brazil., de Campos EC; Faculdade de Medicina FMUSP, Universidade de São Paulo, São Paulo, Brazil., Camargo LDN; Faculdade de Medicina FMUSP, Universidade de São Paulo, São Paulo, Brazil., Dos Santos TM; Faculdade de Medicina FMUSP, Universidade de São Paulo, São Paulo, Brazil., Moreira Bezerra SK; Faculdade de Medicina FMUSP, Universidade de São Paulo, São Paulo, Brazil., de Almeida FM; Faculdade de Medicina FMUSP, Universidade de São Paulo, São Paulo, Brazil., Saraiva-Romanholo BM; Faculdade de Medicina FMUSP, Universidade de São Paulo, São Paulo, Brazil.; University City of São Paulo, São Paulo, Brazil., Lopes FDTQDS; Faculdade de Medicina FMUSP, Universidade de São Paulo, São Paulo, Brazil., Bonturi CR; Departamento de Bioquímica, Universidade Federal de Sao Paulo (UNIFESP), São Paulo, Brazil., Righetti RF; Faculdade de Medicina FMUSP, Universidade de São Paulo, São Paulo, Brazil.; Hospital Sírio-Libanês, São Paulo, Brazil., Oliva MLV; Departamento de Bioquímica, Universidade Federal de Sao Paulo (UNIFESP), São Paulo, Brazil., Tibério IFLC; Faculdade de Medicina FMUSP, Universidade de São Paulo, São Paulo, Brazil., Leick EA; Faculdade de Medicina FMUSP, Universidade de São Paulo, São Paulo, Brazil.
Jazyk:	angličtina
Zdroj:	Frontiers in pharmacology [Front Pharmacol] 2024 May 07; Vol. 15, pp. 1282870. Date of Electronic Publication: 2024 May 07 (Print Publication: 2024).
DOI:	10.3389/fphar.2024.1282870
Abstrakt:	The peptide derived from E. contortisiliquum trypsin inhibitor (Pep-3-EcTI), peptide derived from kallikrein inhibitor isolated from B. bauhinioides (Pep-BbKI), and B. rufa peptide modified from B. bauhinioides (Pep-BrTI) peptides exhibit anti-inflammatory and antioxidant activities, suggesting their potential for treating asthma-chronic obstructive pulmonary disease (COPD) overlap (ACO). We compared the effects of these peptides with dexamethasone (DX) treatment in an ACO model. In this study, 11 groups of male BALB/c mice were pre-treated under different conditions, including sensitization with intraperitoneal injection and inhalation of ovalbumin (OVA), intratracheal instillation of porcine pancreatic elastase (ELA), sensitization with intraperitoneal injection, and various combinations of peptide treatments with Pep-3-EcTI, Pep-BbKI, Pep-BrTI, dexamethasone, and non-treated controls (SAL-saline). Respiratory system resistance, airway resistance, lung tissue resistance, exhaled nitric oxide, linear mean intercept, immune cell counts in the bronchoalveolar lavage fluid, cytokine expression, extracellular matrix remodeling, and oxidative stress in the airways and alveolar septa were evaluated on day 28. Results showed increased respiratory parameters, inflammatory markers, and tissue remodeling in the ACO group compared to controls. Treatment with the peptides or DX attenuated or reversed these responses, with the peptides showing effectiveness in controlling hyperresponsiveness, inflammation, remodeling, and oxidative stress markers. These peptides demonstrated an efficacy comparable to that of corticosteroids in the ACO model. However, this study highlights the need for further research to assess their safety, mechanisms of action, and potential translation to clinical studies before considering these peptides for human use. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 João, Silva Barbosa, Sales da Silva, Fukuzaki, de Campos, Camargo, Santos, Moreira Bezerra, de Almeida, Saraiva-Romanholo, Lopes, Bonturi, Righetti, Oliva, Tibério and Leick.)
Databáze:	MEDLINE
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