LINE-1 retrotransposons contribute to mouse PV interneuron development.

Autor:	Bodea GO; Queensland Brain Institute, University of Queensland, Brisbane, Queensland, Australia. gabriela.bodea@gmail.com.; Mater Research Institute - University of Queensland, TRI Building, Woolloongabba, Queensland, Australia. gabriela.bodea@gmail.com., Botto JM; Queensland Brain Institute, University of Queensland, Brisbane, Queensland, Australia., Ferreiro ME; Queensland Brain Institute, University of Queensland, Brisbane, Queensland, Australia., Sanchez-Luque FJ; Institute of Parasitology and Biomedicine 'López-Neyra', Spanish National Research Council, Granada, Spain., de Los Rios Barreda J; Queensland Brain Institute, University of Queensland, Brisbane, Queensland, Australia., Rasmussen J; Queensland Brain Institute, University of Queensland, Brisbane, Queensland, Australia., Rahman MA; Queensland Brain Institute, University of Queensland, Brisbane, Queensland, Australia., Fenlon LR; Queensland Brain Institute, University of Queensland, Brisbane, Queensland, Australia., Jansz N; Mater Research Institute - University of Queensland, TRI Building, Woolloongabba, Queensland, Australia., Gubert C; Florey Institute of Neuroscience and Mental Health, University of Melbourne, Parkville, Victoria, Australia., Gerdes P; Mater Research Institute - University of Queensland, TRI Building, Woolloongabba, Queensland, Australia., Bodea LG; Clem Jones Centre for Ageing Dementia Research, Queensland Brain Institute, University of Queensland, Brisbane, Queensland, Australia., Ajjikuttira P; Queensland Brain Institute, University of Queensland, Brisbane, Queensland, Australia., Da Costa Guevara DJ; Queensland Brain Institute, University of Queensland, Brisbane, Queensland, Australia.; Mater Research Institute - University of Queensland, TRI Building, Woolloongabba, Queensland, Australia., Cumner L; Queensland Brain Institute, University of Queensland, Brisbane, Queensland, Australia., Bell CC; Mater Research Institute - University of Queensland, TRI Building, Woolloongabba, Queensland, Australia., Kozulin P; Queensland Brain Institute, University of Queensland, Brisbane, Queensland, Australia., Billon V; Queensland Brain Institute, University of Queensland, Brisbane, Queensland, Australia.; Biology Department, École Normale Supérieure Paris-Saclay, Gif-sur-Yvette, France., Morell S; Mater Research Institute - University of Queensland, TRI Building, Woolloongabba, Queensland, Australia.; Department of Genetics, University of Cambridge, Cambridge, UK., Kempen MHC; MRC Human Genetics Unit, Institute of Genetics and Cancer, University of Edinburgh, Western General Hospital, Edinburgh, UK., Love CJ; Florey Institute of Neuroscience and Mental Health, University of Melbourne, Parkville, Victoria, Australia., Saha K; Department of Pharmaceutical Sciences, South Dakota State University, Brookings, SD, USA., Palmer LM; Florey Institute of Neuroscience and Mental Health, University of Melbourne, Parkville, Victoria, Australia.; Florey Department of Neuroscience and Mental Health, University of Melbourne, Parkville, Victoria, Australia., Ewing AD; Mater Research Institute - University of Queensland, TRI Building, Woolloongabba, Queensland, Australia., Jhaveri DJ; Queensland Brain Institute, University of Queensland, Brisbane, Queensland, Australia.; Mater Research Institute - University of Queensland, TRI Building, Woolloongabba, Queensland, Australia., Richardson SR; Mater Research Institute - University of Queensland, TRI Building, Woolloongabba, Queensland, Australia., Hannan AJ; Florey Institute of Neuroscience and Mental Health, University of Melbourne, Parkville, Victoria, Australia., Faulkner GJ; Queensland Brain Institute, University of Queensland, Brisbane, Queensland, Australia. faulknergj@gmail.com.; Mater Research Institute - University of Queensland, TRI Building, Woolloongabba, Queensland, Australia. faulknergj@gmail.com.
Jazyk:	angličtina
Zdroj:	Nature neuroscience [Nat Neurosci] 2024 Jul; Vol. 27 (7), pp. 1274-1284. Date of Electronic Publication: 2024 May 21.
DOI:	10.1038/s41593-024-01650-2
Abstrakt:	Retrotransposons are mobile DNA sequences duplicated via transcription and reverse transcription of an RNA intermediate. Cis-regulatory elements encoded by retrotransposons can also promote the transcription of adjacent genes. Somatic LINE-1 (L1) retrotransposon insertions have been detected in mammalian neurons. It is, however, unclear whether L1 sequences are mobile in only some neuronal lineages or therein promote neurodevelopmental gene expression. Here we report programmed L1 activation by SOX6, a transcription factor critical for parvalbumin (PV) interneuron development. Mouse PV interneurons permit L1 mobilization in vitro and in vivo, harbor unmethylated L1 promoters and express full-length L1 mRNAs and proteins. Using nanopore long-read sequencing, we identify unmethylated L1s proximal to PV interneuron genes, including a novel L1 promoter-driven Caps2 transcript isoform that enhances neuron morphological complexity in vitro. These data highlight the contribution made by L1 cis-regulatory elements to PV interneuron development and transcriptome diversity, uncovered due to L1 mobility in this milieu. (© 2024. The Author(s).)
Databáze:	MEDLINE
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