Imaging the extracellular matrix in live tissues and organisms with a glycan-binding fluorophore.

Autor:	Fiore A; Janelia Research Campus, Howard Hughes Medical Institute (HHMI), Ashburn, VA, USA., Yu G; Janelia Research Campus, Howard Hughes Medical Institute (HHMI), Ashburn, VA, USA., Northey JJ; Center for Bioengineering and Tissue Regeneration, Department of Surgery, University of California, San Francisco (UCSF), San Francisco, CA, USA., Patel R; Janelia Research Campus, Howard Hughes Medical Institute (HHMI), Ashburn, VA, USA., Ravenscroft TA; Janelia Research Campus, Howard Hughes Medical Institute (HHMI), Ashburn, VA, USA., Ikegami R; Janelia Research Campus, Howard Hughes Medical Institute (HHMI), Ashburn, VA, USA., Kolkman W; Janelia Research Campus, Howard Hughes Medical Institute (HHMI), Ashburn, VA, USA., Kumar P; Janelia Research Campus, Howard Hughes Medical Institute (HHMI), Ashburn, VA, USA., Grimm JB; Janelia Research Campus, Howard Hughes Medical Institute (HHMI), Ashburn, VA, USA., Dilan TL; Janelia Research Campus, Howard Hughes Medical Institute (HHMI), Ashburn, VA, USA., Ruetten VMS; Janelia Research Campus, Howard Hughes Medical Institute (HHMI), Ashburn, VA, USA., Ahrens MB; Janelia Research Campus, Howard Hughes Medical Institute (HHMI), Ashburn, VA, USA., Shroff H; Janelia Research Campus, Howard Hughes Medical Institute (HHMI), Ashburn, VA, USA., Lavis LD; Janelia Research Campus, Howard Hughes Medical Institute (HHMI), Ashburn, VA, USA., Wang S; Janelia Research Campus, Howard Hughes Medical Institute (HHMI), Ashburn, VA, USA., Weaver VM; Center for Bioengineering and Tissue Regeneration, Department of Surgery, University of California, San Francisco (UCSF), San Francisco, CA, USA., Pedram K; Janelia Research Campus, Howard Hughes Medical Institute (HHMI), Ashburn, VA, USA.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2024 May 10. Date of Electronic Publication: 2024 May 10.
DOI:	10.1101/2024.05.09.593460
Abstrakt:	All multicellular systems produce and dynamically regulate extracellular matrices (ECM) that play important roles in both biochemical and mechanical signaling. Though the spatial arrangement of these extracellular assemblies is critical to their biological functions, visualization of ECM structure is challenging, in part because the biomolecules that compose the ECM are difficult to fluorescently label individually and collectively. Here, we present a cell-impermeable small molecule fluorophore, termed Rhobo6, that turns on and red shifts upon reversible binding to glycans. Given that most ECM components are densely glycosylated, the dye enables wash-free visualization of ECM, in systems ranging from in vitro substrates to in vivo mouse mammary tumors. Relative to existing techniques, Rhobo6 provides a broad substrate profile, superior tissue penetration, nonperturbative labeling, and negligible photobleaching. This work establishes a straightforward method for imaging the distribution of ECM in live tissues and organisms, lowering barriers for investigation of extracellular biology. Competing Interests: Competing Interests A patent application relating to this work has been filed by the Howard Hughes Medical Institute (internal reference 2024-017-01).
Databáze:	MEDLINE
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