Electrochemical Detection of Melphalan in Biological Fluids Using a g-C 3 N 4 @ND-COOH@MoSe 2 Modified Electrode Complemented by Molecular Docking Studies with Cellular Tumor Antigen P53.

Autor: Erk N; Ankara University, Faculty of Pharmacy, Department of Analytical Chemistry, 06560 Ankara, Turkey., Kurtay G; Hacettepe University, Faculty of Sciences, Department of Chemistry, 06800 Ankara, Turkey., Bouali W; Ankara University, Faculty of Pharmacy, Department of Analytical Chemistry, 06560 Ankara, Turkey.; Ankara University, Graduate School of Health Sciences, 06110 Ankara, Turkey., Sakal ZG; Ankara University, Faculty of Pharmacy, Department of Analytical Chemistry, 06560 Ankara, Turkey.; Ankara University, Graduate School of Health Sciences, 06110 Ankara, Turkey., Genç AA; Ankara University, Faculty of Pharmacy, Department of Analytical Chemistry, 06560 Ankara, Turkey.; Ankara University, Graduate School of Health Sciences, 06110 Ankara, Turkey., Erbaş Z; Yozgat Bozok University, Science and Technology Application and Research Center, 66200 Yozgat, Turkey.; Erciyes University, Technology Research & Application Center (TAUM), 38039 Kayseri, Turkey., Soylak M; Erciyes University, Technology Research & Application Center (TAUM), 38039 Kayseri, Turkey.; Turkish Academy of Sciences (TUBA), Çankaya, Ankara 06670, Turkey.
Jazyk: angličtina
Zdroj: ACS omega [ACS Omega] 2024 Apr 30; Vol. 9 (19), pp. 21058-21070. Date of Electronic Publication: 2024 Apr 30 (Print Publication: 2024).
DOI: 10.1021/acsomega.4c00558
Abstrakt: Melphalan (Mel) is a potent alkylating agent utilized in chemotherapy treatments for a diverse range of malignancies. The need for its accurate and timely detection in pharmaceutical preparations and biological samples is paramount to ensure optimized therapeutic efficacy and to monitor treatment progression. To address this critical need, our study introduced a cutting-edge electrochemical sensor. This device boasts a uniquely modified electrode crafted from graphitic carbon nitride (g-C 3 N 4 ), decorated with activated nanodiamonds (ND-COOH) and molybdenum diselenide (MoSe 2 ), and specifically designed to detect Mel with unparalleled precision. Our rigorous testing employed advanced techniques such as cyclic voltammetry and differential pulse voltammetry. The outcomes were promising; the sensor consistently exhibited a linear response in the range of 0.5 to 12.5 μM. Even more impressively, the detection threshold was as low as 0.03 μM, highlighting its sensitivity. To further enhance our understanding of Mel's biological interactions, we turned to molecular docking studies. These studies primarily focused on Mel's interaction dynamics with the cellular tumor antigen P53, revealing a binding affinity of -5.0 kcal/mol. A fascinating observation was made when Mel was covalently conjugated with nanodiamond-COOH (ND-COOH). This conjugation resulted in a binding affinity that surged to -10.9 kcal/mol, clearly underscoring our sensor's superior detection capabilities. This observation also reinforced the wisdom behind incorporating ND-COOH in our electrode design. In conclusion, our sensor not only stands out in terms of sensitivity but also excels in selectivity and accuracy. By bridging electrochemical sensing with computational insights, our study illuminates Mel's intricate behavior, driving advancements in sensor technology and potentially revolutionizing cancer therapeutic strategies.
Competing Interests: The authors declare no competing financial interest.
(© 2024 The Authors. Published by American Chemical Society.)
Databáze: MEDLINE
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