Achyrocline satureioides infusion, popularly prepared and consumed, has an in vitro protective effect on human neural cells exposed to rotenone.

Autor: Mânica da Cruz IB; Laboratório Biogenômica, Universidade Federal de Santa Maria, Avenida Roraima, 1000, 97105-900, Santa Maria, RS, Brazil; Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria, Avenida Roraima, 1000, 97105-900, Santa Maria, RS, Brazil; Programa de Pós-Graduação em Gerontologia, Universidade Federal de Santa Maria, Avenida Roraima, 1000, 97105-900, Santa Maria, RS, Brazil., Chelotti ME; Laboratório Biogenômica, Universidade Federal de Santa Maria, Avenida Roraima, 1000, 97105-900, Santa Maria, RS, Brazil., Turra BO; Laboratório Biogenômica, Universidade Federal de Santa Maria, Avenida Roraima, 1000, 97105-900, Santa Maria, RS, Brazil; Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria, Avenida Roraima, 1000, 97105-900, Santa Maria, RS, Brazil., Cardoso de Afonso Bonotto N; Laboratório Biogenômica, Universidade Federal de Santa Maria, Avenida Roraima, 1000, 97105-900, Santa Maria, RS, Brazil; Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria, Avenida Roraima, 1000, 97105-900, Santa Maria, RS, Brazil., Pulcinelli DF; Laboratório Biogenômica, Universidade Federal de Santa Maria, Avenida Roraima, 1000, 97105-900, Santa Maria, RS, Brazil., Kerkhoff Escher AL; Laboratório Biogenômica, Universidade Federal de Santa Maria, Avenida Roraima, 1000, 97105-900, Santa Maria, RS, Brazil., Klein C; Laboratório Biogenômica, Universidade Federal de Santa Maria, Avenida Roraima, 1000, 97105-900, Santa Maria, RS, Brazil., de Azevedo Mello P; Departamento de Química, Universidade Federal de Santa Maria, Avenida Roraima, 1000, 97105-900, Santa Maria, RS, Brazil; Programa de Pós-Graduação em Química, Universidade Federal de Santa Maria, Avenida Roraima, 1000, 97105-900, Santa Maria, RS, Brazil., Bitencourt GR; Programa de Pós-Graduação em Química, Universidade Federal de Santa Maria, Avenida Roraima, 1000, 97105-900, Santa Maria, RS, Brazil., Barbisan F; Laboratório Biogenômica, Universidade Federal de Santa Maria, Avenida Roraima, 1000, 97105-900, Santa Maria, RS, Brazil; Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria, Avenida Roraima, 1000, 97105-900, Santa Maria, RS, Brazil; Programa de Pós-Graduação em Gerontologia, Universidade Federal de Santa Maria, Avenida Roraima, 1000, 97105-900, Santa Maria, RS, Brazil. Electronic address: fernandabarbisan@gmail.com.
Jazyk: angličtina
Zdroj: Journal of ethnopharmacology [J Ethnopharmacol] 2024 Oct 05; Vol. 332, pp. 118350. Date of Electronic Publication: 2024 May 17.
DOI: 10.1016/j.jep.2024.118350
Abstrakt: Ethnopharmacological Relevance: Traditional harvest of Achyrocline satureioides (AS) occurs at dawn on Good Friday in some South American countries. Inflorescences are traditionally used as infusions for several disorders, including neuropsychiatric disorders. Pillows and cushions are popularly filled with AS to attenuate the symptoms of depression, anxiety, and sleep disturbances. However, evidence for the potential beneficial effects of AS on human neural cells remains unclear.
Aim of the Study: An in vitro model of SH-SY5Y human neural cells was applied to evaluate the effect of AS infusion, prepared as commonly used, on cells exposed to rotenone and to investigate its potential for neuropsychiatric disorders.
Materials and Methods: A hot aqueous extract was obtained from a traditionally prepared AS inflorescence infusion and chemically characterized by high-resolution mass spectrometry and spectrophotometric quantification of total polyphenols, tannins, and flavonoids. The SH-SY5Y cell cultures were treated with AS extract at concentrations of 1, 3, 5, 10, 50, 100, and 300 μL/mL to determine the potential cyto- and genotoxic effects of AS on neural cells using MTT, Neutral Red, and GEMO assays. Apoptosis modulation was assessed using flow cytometry and apoptosis-modulating genes were evaluated by qRT-PCR. The protective effect of AS on the neurotoxicity triggered by rotenone exposure (30 nM) was determined by analyzing cellular viability and oxidative markers such as lipid peroxidation and protein carbonylation, and DNA damage was assessed by micronucleus assay.
Results: The AS extract, as traditionally prepared, had estimated concentrations of 409.973 ± 31.107 μg/mL, 0.1041 ± 0.0246 mg GAE/mL, and 63.309 ± 3.178 mg QE/mL of total tannins, total polyphenols, and flavonoids, respectively. At concentrations of 30 and 100 μl/mL, AS decreased apoptotic events, whereas the highest concentration (300 μl/mL) increased apoptosis compared to that in the control (p < 0.05). In cells exposed to rotenone, AS treatment induced cell proliferation, reduced DNA damage (as evaluated by micronuclei), and reduced lipid and protein oxidation.
Conclusions: The data indicate the non-cytotoxic and beneficial effects of AS extract on human neural cells by reducing cellular mortality and oxidative stress in neural cells triggered by rotenone exposure.
Competing Interests: Declaration of competing interest The authors declare no conflict of interest in this manuscript.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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