A tonoplast-localized TPK-type K + transporter (TPKa) regulates potassium accumulation in tobacco.

Autor: Gao Y; Yunnan Academy of Tobacco Agricultural Sciences/National Tobacco Genetic Engineering Research Center, Kunming, China., Zhao L; Yunnan Academy of Tobacco Agricultural Sciences/National Tobacco Genetic Engineering Research Center, Kunming, China., Wang B; Yunnan Academy of Tobacco Agricultural Sciences/National Tobacco Genetic Engineering Research Center, Kunming, China., Song Z; Yunnan Academy of Tobacco Agricultural Sciences/National Tobacco Genetic Engineering Research Center, Kunming, China., Jiao F; Yunnan Academy of Tobacco Agricultural Sciences/National Tobacco Genetic Engineering Research Center, Kunming, China., Wu X; Yunnan Academy of Tobacco Agricultural Sciences/National Tobacco Genetic Engineering Research Center, Kunming, China., Feng Z; Yunnan Academy of Tobacco Agricultural Sciences/National Tobacco Genetic Engineering Research Center, Kunming, China., Chen X; Yunnan Academy of Tobacco Agricultural Sciences/National Tobacco Genetic Engineering Research Center, Kunming, China., Gao L; Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China. Electronic address: gaolifeng@caas.cn., Li Y; Yunnan Academy of Tobacco Agricultural Sciences/National Tobacco Genetic Engineering Research Center, Kunming, China. Electronic address: 309349240@qq.com.
Jazyk: angličtina
Zdroj: Gene [Gene] 2024 Oct 30; Vol. 926, pp. 148576. Date of Electronic Publication: 2024 May 17.
DOI: 10.1016/j.gene.2024.148576
Abstrakt: Potassium ion (K + ) is one of the most essential nutrients for the growth and development of tobacco (Nicotiana tabacum L.), however, the molecular regulation of K + concentration in tobacco remains unclear. In this study, a two-pore K (TPK) channel gene NtTPKa was cloned from tobacco, and NtTPKa protein contains the unique K + selection motif GYGD and its transmembrane region primarily locates in the tonoplast membrane. The expression of NtTPKa gene was significantly increased under low-potassium stress conditions. The concentrations of K + in tobacco were significantly increased in the NtTPKa RNA interference lines and CRISPR/Cas9 knockout mutants. In addition, the transport of K + by NtTPKa was validated using patch clamp technique, and the results showed that NtTPKa channel protein exclusively transported K + in a concentration-dependent manner. Together, our results strongly suggested that NtTPKa is a key gene in maintaining K + homeostasis in tobacco, and it could provide a new genetic resource for increasing the concentration of K + in tobacco.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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