| Autor: |
Zhang Y; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, USA.; John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts, USA. yizhe_zhang@post.harvard.edu., Agresti JJ; John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts, USA. yizhe_zhang@post.harvard.edu., Zheng Y; New England BioLabs, Inc., Ipswich, MA 01938, USA., Weitz DA; John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts, USA. yizhe_zhang@post.harvard.edu.; Department of Physics, Harvard University, Cambridge, MA 02138, USA. |
| Abstrakt: |
A restriction endonuclease (RE) is an enzyme that can recognize a specific DNA sequence and cleave that DNA into fragments with double-stranded breaks. This sequence-specific cleaving ability and its ease of use have made REs commonly used tools in molecular biology since their first isolation and characterization in 1970s. While artificial REs still face many challenges in large-scale synthesis and precise activity control for practical use, searching for new REs in natural samples remains a viable route to expanding the RE pool for fundamental research and industrial applications. In this paper, we propose a new strategy to search for REs in an efficient manner. We constructed a host bacterial cell to link the genotype of REs to the phenotype of β-galactosidase expression based on the bacterial SOS response, and used a high-throughput microfluidic platform to isolate, detect and sort the REs in microfluidic drops at a frequency of ∼800 drops per second. We employed this strategy to screen for the XbaI gene from the constructed libraries of varied sizes. In a single round of sorting, a 90-fold target enrichment was achieved within 1 h. Compared to conventional RE-screening methods, the direct screening approach that we propose excels at efficient search of desirable REs in natural samples - especially unculturable samples - and can be tailored to high-throughput screening of a wide range of genotoxic targets. |
