Plasma Virome of HIV-infected Subjects on Suppressive Antiretroviral Therapy Reveals Association of Differentially Abundant Viruses with Distinct T-cell Phenotypes and Inflammation.

Autor:	Bhagchandani T; Laboratory of AIDS Research and Immunology, School of Biotechnology, Jawaharlal Nehru University, New Delhi, India., Haque MMU; School of Computational and Integrative Sciences, Jawaharlal Nehru University, New Delhi, India., Sharma S; School of Biotechnology, Jawaharlal Nehru University, New Delhi, India., Malik MZ; Host-Pathogen Interaction Laboratory, School of Biotechnology, Jawaharlal Nehru University, New Delhi, India., Ray AK; Laboratory of Metabolic Disorder and Environmental Biotechnology, Department of Environmental Studies, Faculty of Science, University of Delhi, New Delhi, India., Kaur US; Laboratory of AIDS Research and Immunology, School of Biotechnology, Jawaharlal Nehru University, New Delhi, India., Rai A; Laboratory of AIDS Research and Immunology, School of Biotechnology, Jawaharlal Nehru University, New Delhi, India., Verma A; Laboratory of AIDS Research and Immunology, School of Biotechnology, Jawaharlal Nehru University, New Delhi, India., Sawlani KK; Department of Medicine, King George's Medical University, Lucknow, India., Chaturvedi R; Host-Pathogen Interaction Laboratory, School of Biotechnology, Jawaharlal Nehru University, New Delhi, India.; Special Centre for System Medicine, Jawaharlal Nehru University, New Delhi, India., Dandu H; Department of Medicine, King George's Medical University, Lucknow, India., Kumar A; Institute of Bioinformatics, International Technology Park, Bangalore; India.; Manipal Academy of Higher Education (MAHE), Manipal, India., Tandon R; Laboratory of AIDS Research and Immunology, School of Biotechnology, Jawaharlal Nehru University, New Delhi, India.
Jazyk:	angličtina
Zdroj:	Current genomics [Curr Genomics] 2024 Apr 08; Vol. 25 (2), pp. 105-119.
DOI:	10.2174/0113892029279786240111052824
Abstrakt:	Background: The plasma virome represents the overall composition of viral sequences present in it. Alteration in plasma virome has been reported in treatment naïve and immunocompromised (CD4 count < 200) people with HIV (PWH). However, the effect of ART on virome composition in PWH on ART with preserved CD4 counts is poorly understood. Objectives: We aimed to assess the alterations in plasma virome in PWH on ART in comparison to HIV-negative uninfected controls and to further investigate possible associations of plasma viruses with inflammation and immune dysfunction, namely, immunosenescence and immune exhaustion. Methods: Plasma viral DNA from PWH on ART and controls was used for sequencing on the Illumina Nextseq500 platform, followed by the identification of viral sequences using an automated pipeline, VIROMATCH. Multiplex cytokine assay was performed to measure the concentrations of various cytokines in plasma. Immunophenotyping was performed on PBMCs to identify T cell markers of immunosenescence and immune exhaustion. Results: In our observational, cross-sectional pilot study, chronically infected PWH on ART had significantly different viral species compositions compared to controls. The plasma virome of PWH showed a significantly high relative abundance of species Human gammaherpesvirus 4, also known as Epstein-Barr virus (EBV). Moreover, EBV emerged as a significant viral taxon differentially enriched in PWH on ART, which further correlated positively with the exhaustion phenotype of T cells and significantly increased TNF-α in PWH on ART. Additionally, a significantly increased proportion of senescent T cells and IL-8 cytokine was detected in PWH on ART. Conclusion: Altered plasma virome influenced the inflammatory response and T-cell phenotype in PWH on ART. Competing Interests: The authors declare no conflict of interest, financial or otherwise. (© 2024 Bentham Science Publishers.)
Databáze:	MEDLINE
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