Granular Biphasic Colloidal Hydrogels for 3D Bioprinting.

Autor: Deo KA; Biomedical Engineering, College of Engineering, Texas A&M University, College Station, TX, 77843, USA., Murali A; Biomedical Engineering, College of Engineering, Texas A&M University, College Station, TX, 77843, USA., Tronolone JJ; Biomedical Engineering, College of Engineering, Texas A&M University, College Station, TX, 77843, USA., Mandrona C; Biomedical Engineering, College of Engineering, Texas A&M University, College Station, TX, 77843, USA., Lee HP; Biomedical Engineering, College of Engineering, Texas A&M University, College Station, TX, 77843, USA., Rajput S; Biomedical Engineering, College of Engineering, Texas A&M University, College Station, TX, 77843, USA., Hargett SE; Biomedical Engineering, College of Engineering, Texas A&M University, College Station, TX, 77843, USA., Selahi A; Biomedical Engineering, College of Engineering, Texas A&M University, College Station, TX, 77843, USA., Sun Y; Nutrition, College of Agriculture, Texas A&M University, College Station, TX, 77843, USA., Alge DL; Biomedical Engineering, College of Engineering, Texas A&M University, College Station, TX, 77843, USA.; Material Science and Engineering, College of Engineering, Texas A&M University, College Station, TX, 77843, USA., Jain A; Biomedical Engineering, College of Engineering, Texas A&M University, College Station, TX, 77843, USA.; Medical Physiology, School of Medicine, Texas A&M Health Science Center, Bryan, TX, USA.; Cardiovascular Sciences, Houston Methodist Research Institute, Houston, TX, 77030, USA., Gaharwar AK; Biomedical Engineering, College of Engineering, Texas A&M University, College Station, TX, 77843, USA.; Material Science and Engineering, College of Engineering, Texas A&M University, College Station, TX, 77843, USA.; Cardiovascular Sciences, Houston Methodist Research Institute, Houston, TX, 77030, USA.; Interdisciplinary Graduate Program in Genetics & Genomics, Texas A&M University, College Station, TX, 77843, USA.; Center for Remote Health Technologies and Systems, Texas A&M University, College Station, TX, 77843, USA.
Jazyk: angličtina
Zdroj: Advanced healthcare materials [Adv Healthc Mater] 2024 Oct; Vol. 13 (25), pp. e2303810. Date of Electronic Publication: 2024 May 29.
DOI: 10.1002/adhm.202303810
Abstrakt: Granular hydrogels composed of hydrogel microparticles are promising candidates for 3D bioprinting due to their ability to protect encapsulated cells. However, to achieve high print fidelity, hydrogel microparticles need to jam to exhibit shear-thinning characteristics, which is crucial for 3D printing. Unfortunately, this overpacking can significantly impact cell viability, thereby negating the primary advantage of using hydrogel microparticles to shield cells from shear forces. To overcome this challenge, a novel solution: a biphasic, granular colloidal bioink designed to optimize cell viability and printing fidelity is introduced. The biphasic ink consists of cell-laden polyethylene glycol (PEG) hydrogel microparticles embedded in a continuous gelatin methacryloyl (GelMA)-nanosilicate colloidal network. Here, it is demonstrated that this biphasic bioink offers outstanding rheological properties, print fidelity, and structural stability. Furthermore, its utility for engineering complex tissues with multiple cell types and heterogeneous microenvironments is demonstrated, by incorporating β-islet cells into the PEG microparticles and endothelial cells in the GelMA-nanosilicate colloidal network. Using this approach, it is possible to induce cell patterning, enhance vascularization, and direct cellular function. The proposed biphasic bioink holds significant potential for numerous emerging biomedical applications, including tissue engineering and disease modeling.
(© 2024 The Author(s). Advanced Healthcare Materials published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
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