Francisella salimarina infection in farmed cobia (Rachycentron canadum) and dusky grouper (Epinephelus marginatus) in Brazil: A case report.

Autor: Rocha VP; Department of Preventive Veterinary Medicine, School of Veterinary Medicine, Federal University of Minas Gerais - UFMG, Belo Horizonte, Minas Gerais, Brazil., Gauer D; Department of Preventive Veterinary Medicine, School of Veterinary Medicine, Federal University of Minas Gerais - UFMG, Belo Horizonte, Minas Gerais, Brazil., Costa HL; Department of Preventive Veterinary Medicine, School of Veterinary Medicine, Federal University of Minas Gerais - UFMG, Belo Horizonte, Minas Gerais, Brazil., Reis FYT; Department of Preventive Veterinary Medicine, School of Veterinary Medicine, Federal University of Minas Gerais - UFMG, Belo Horizonte, Minas Gerais, Brazil., Marcelino SAC; Department of Veterinary Clinics and Surgery, School of Veterinary Medicine, Federal University of Minas Gerais - UFMG, Belo Horizonte, Minas Gerais, Brazil., Nogueira LFF; Department of Preventive Veterinary Medicine, School of Veterinary Medicine, Federal University of Minas Gerais - UFMG, Belo Horizonte, Minas Gerais, Brazil., Pierezan F; Department of Veterinary Clinics and Surgery, School of Veterinary Medicine, Federal University of Minas Gerais - UFMG, Belo Horizonte, Minas Gerais, Brazil., Figueiredo HCP; Department of Preventive Veterinary Medicine, School of Veterinary Medicine, Federal University of Minas Gerais - UFMG, Belo Horizonte, Minas Gerais, Brazil., Tavares GC; Department of Preventive Veterinary Medicine, School of Veterinary Medicine, Federal University of Minas Gerais - UFMG, Belo Horizonte, Minas Gerais, Brazil.
Jazyk: angličtina
Zdroj: Journal of fish diseases [J Fish Dis] 2024 May 15, pp. e13962. Date of Electronic Publication: 2024 May 15.
DOI: 10.1111/jfd.13962
Abstrakt: This study aimed to identify and characterize isolates of Francisella salimarina associated with an outbreak on a marine fish farm in Brazil and to analyse their genetic variability and antimicrobial susceptibility. In 2021, diseased cobias (Rachycentron canadum, n = 10) and dusky groupers (Epinephelus marginatus, n = 10) were sampled and subjected to bacteriological and pathological examinations. The isolates obtained were morphologically and biochemically characterized and identified by matrix-assisted laser desorption/ionization-time of flight (MALDI-ToF) and 16S rRNA gene sequencing. The genetic diversity of these isolates was analysed using repetitive extragenic palindromic-polymerase chain reaction (REP-PCR). Antimicrobial susceptibility was assessed using the disk diffusion technique. Macroscopically, the fish presented skin ulcerations, ocular lesions, hepatomegaly and splenomegaly. A pleomorphic, gram-negative, catalase- and oxidase-positive bacterium was isolated from seven cobias and two groupers. The 16S rRNA gene sequences showed >99% coverage and identity with other deposited sequences of F. salimarina. The results of the biochemical analysis corresponded to these bacterial species. Histologically, granulomas were observed in the spleen, liver and heart of the cobias (n = 6), and necrotizing and fibrinous dermatitis and myositis were identified in some groupers (n = 2). The isolates exhibited the same banding pattern when REP-PCR was performed, indicating that they were clonally related. Finally, the antibiogram test, no inhibition halo was observed for amoxicillin and trimethoprim-sulfamethoxazole. To our knowledge, this is the first report of F. salimarina infection in cobias and dusky groupers.
(© 2024 John Wiley & Sons Ltd.)
Databáze: MEDLINE
Externí odkaz: