| Autor: |
Stapf CA; Program in Neuroscience, University of Maryland Baltimore, Baltimore, MD, 21201.; Department of Neurobiology, University of Maryland School of Medicine, Baltimore, MD 21201., Keefer SE; Department of Neurobiology, University of Maryland School of Medicine, Baltimore, MD 21201., McInerney JM; Program in Neuroscience, University of Maryland Baltimore, Baltimore, MD, 21201.; Department of Neurobiology, University of Maryland School of Medicine, Baltimore, MD 21201., Cheer JF; Program in Neuroscience, University of Maryland Baltimore, Baltimore, MD, 21201.; Department of Neurobiology, University of Maryland School of Medicine, Baltimore, MD 21201., Calu DJ; Program in Neuroscience, University of Maryland Baltimore, Baltimore, MD, 21201.; Department of Neurobiology, University of Maryland School of Medicine, Baltimore, MD 21201. |
| Abstrakt: |
Cannabinoid-1 receptor (CB1R) signaling in the dorsal striatum regulates the shift from flexible to habitual behavior in instrumental outcome devaluation. Based on prior work establishing individual, sex, and experience-dependent differences in Pavlovian behaviors, we predicted a role for dorsomedial striatum CB1R signaling in driving rigid responding in Pavlovian autoshaping and outcome devaluation. We trained male and female Long Evans rats in Pavlovian Lever Autoshaping (PLA). We gave intra-dorsomedial striatum (DMS) infusions of the CB1R inverse agonist, rimonabant, before satiety-induced outcome devaluation test sessions, where we sated rats on training pellets or home cage chow and tested them in brief nonreinforced Pavlovian Lever Autoshaping sessions. Overall, inhibition of DMS CB1R signaling prevented Pavlovian outcome devaluation but did not affect behavior in reinforced PLA sessions. Males were sensitive to devaluation while females were not and DMS CB1R inhibition impaired devaluation sensitivity in males. We then investigated how DMS CB1R signaling impacts local inhibitory synaptic transmission in male and female Long Evans rats. We recorded spontaneous inhibitory postsynaptic currents (sIPSC) from DMS neurons at baseline and before and after application of a CB1R agonist, WIN 55,212-2. We found that male rats showed decreased sIPSC frequency compared to females, and that CB1R activation reduced DMS inhibitory transmission independent of sex. Altogether our results demonstrate that DMS CB1Rs regulate Pavlovian devaluation sensitivity and inhibitory synaptic transmission and suggest that basal sex differences in inhibitory synaptic transmission may underly sex differences in DMS function and behavioral flexibility. |
