Plant-based zinc nanoflowers assisted molecularly imprinted polymer for the design of an electrochemical sensor for selective determination of abrocitinib.

Autor: Cetinkaya A; Department of Analytical Chemistry, Faculty of Pharmacy, Ankara University, Ankara, 06560, Turkey., Yusufbeyoglu S; Department of Pharmaceutical Botany, Gulhane Faculty of Pharmacy, University of Health Sciences, Ankara, Turkey., Kaya SI; Department of Analytical Chemistry, Gulhane Faculty of Pharmacy, University of Health Sciences, Ankara, Turkey., Kilic AB; Department of Pharmaceutical Botany, Gulhane Faculty of Pharmacy, University of Health Sciences, Ankara, Turkey., Atici EB; DEVA Holding A.S., Research&Development Center, Tekirdag, 59510, Turkey., Ozkan SA; Department of Analytical Chemistry, Faculty of Pharmacy, Ankara University, Ankara, 06560, Turkey. ozkan@pharmacy.ankara.edu.tr.
Jazyk: angličtina
Zdroj: Mikrochimica acta [Mikrochim Acta] 2024 May 10; Vol. 191 (6), pp. 322. Date of Electronic Publication: 2024 May 10.
DOI: 10.1007/s00604-024-06404-2
Abstrakt: The first electrochemical sensor application in the literature is described for the sensitive and selective determination of the selective Janus kinase (JAK)-1 inhibitor abrocitinib (ABR). ABR is approved by the U.S. Food and Drug Administration (FDA) for the treatment of atopic dermatitis. The molecularly imprinted polymer (MIP)-based sensor was designed to incorporate zinc nanoflower (ZnNFs)-graphene oxide (GO) conjugate (ZnNFs@GO), synthesized from the root methanolic extract (RME) of the species Alkanna cappadocica Boiss. et Bal. to improve the porosity and effective surface area of the glassy carbon electrode (GCE). Furthermore, the MIP structure was prepared using ABR as a template molecule, 4-aminobenzoic acid (4-ABA) as a functional monomer, and other additional components. Scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR) were used to characterize the surface and structure of the synthesized nanomaterial and MIP-based surface. Among the electrochemical methods, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were preferred for detailed electrochemical characterization, and differential pulse voltammetry (DPV) was preferred for all other electrochemical measurements using 5.0 mM [Fe(CN) 6 ] 3-/4- solution as the redox probe. The MIP-based sensor, which was the result of a detailed optimization phase, gave a linear response in the 1.0 × 10 -13 - 1.0 × 10 -12  M range in standard solution and serum sample. The obtained limit of detection (LOD) and limit of quantification (LOQ) values and recovery studies demonstrated the sensitivity, accuracy, and applicability of the sensor. Selectivity, the most important feature of the MIP-based sensor, was verified by imprinting factor calculations using ibrutinib, ruxolitinib, tofacitinib, zonisamide, and acetazolamide.
(© 2024. The Author(s).)
Databáze: MEDLINE
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