Cytotoxic potential and metabolomic profiling of alkaloid rich fraction of Tylophora indica leaves.

Autor: Kausar MA; Department of Biochemistry, College of Medicine, University of Ha'il, Hail 81411, Saudi Arabia; Medical and Diagnostic Research Centre, University of Ha'il, Hail 55473, Saudi Arabia. Electronic address: ma.kausar@uoh.edu.sa., Parveen S; Department of Biosciences, Jamia Millia Islamia, Okhla, New Delhi 110025, India. Electronic address: mailshabanap@gmail.com., Anwar S; Department of Biochemistry, College of Medicine, University of Ha'il, Hail 81411, Saudi Arabia; Medical and Diagnostic Research Centre, University of Ha'il, Hail 55473, Saudi Arabia. Electronic address: sa.anwar@uoh.edu.sa., Sadaf; Department of Biotechnology, Jamia Millia Islamia, Okhla, New Delhi 110025, India., Massey S; Department of Biosciences, Jamia Millia Islamia, Okhla, New Delhi 110025, India. Electronic address: masseysheersh@gmail.com., El-Horany HE; Department of Biochemistry, College of Medicine, University of Ha'il, Hail 81411, Saudi Arabia; Medical and Diagnostic Research Centre, University of Ha'il, Hail 55473, Saudi Arabia; Medical Biochemistry Department, Faculty of Medicine, Tanta University, Egypt. Electronic address: h.elhorany@uoh.edu.sa., Khan FH; Medical and Diagnostic Research Centre, University of Ha'il, Hail 55473, Saudi Arabia; Department of Community and Family Medicine, College of Medicine, University of Ha'il, Hail 81411, Saudi Arabia. Electronic address: fa.khan@uoh.edu.sa., Shahein M; Department of Pediatrics, College of Medicine, University of Ha'il, Hail 81411, Saudi Arabia., Husain SA; Department of Biosciences, Jamia Millia Islamia, Okhla, New Delhi 110025, India. Electronic address: shusain@jmi.ac.in.
Jazyk: angličtina
Zdroj: Biochimica et biophysica acta. Molecular and cell biology of lipids [Biochim Biophys Acta Mol Cell Biol Lipids] 2024 Aug; Vol. 1869 (6), pp. 159505. Date of Electronic Publication: 2024 May 08.
DOI: 10.1016/j.bbalip.2024.159505
Abstrakt: Tylophora indica (Burm f.) Merrill, belong to family Asclepiadaceae, is considered to be a natural remedy with high medicinal benefits. The objective of this work is to assess the metabolomic profile of T. indica leaves enriched in alkaloids, as well as to evaluate the in vitro cytotoxicity of these leaves using the MTT assay on human breast MCF-7 and liver HepG2 cancer cell lines. Dried leaves of T. indica were extracted by sonication, using methanol containing 2 % (v/v) of acetic acid and obtained fraction was characterized by HPTLC and UPLC-MS. The UPLC-MS study yielded a preliminary identification of 32 metabolites, with tylophorine, tylophorine B, tylophorinine, and tylophorinidine being the predominant metabolites. The cytotoxicity of the extract of T. indica was evaluated on HepG2 and MCF-7 cell lines, yielding inhibitory concentration (IC 50 ) values of 75.71 μg/mL and 69.60 μg/mL, respectively. Data suggested that the phytochemical screening clearly showed presence of numerous secondary metabolites with moderate cytotoxic efficacy. In conclusion, the future prospects of T. indica appear promising for the advancement of phytopharmaceutical-based anticancer medications, as well as for the design of contemporary pharmaceuticals in the field of cancer chemotherapy.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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