Biochemical characterization of Fsa16295Glu from "Fervidibacter sacchari," the first hyperthermophilic GH50 with β-1,3-endoglucanase activity and founding member of the subfamily GH50_3.
| Autor: | Covington JK; School of Life Sciences, University of Nevada, Las Vegas, NV, United States., Torosian N; School of Life Sciences, University of Nevada, Las Vegas, NV, United States., Cook AM; School of Life Sciences, University of Nevada, Las Vegas, NV, United States., Palmer M; School of Life Sciences, University of Nevada, Las Vegas, NV, United States.; Department of Microbiology, University of Manitoba, Winnipeg, MB, Canada., Bryan SG; School of Life Sciences, University of Nevada, Las Vegas, NV, United States., Nou NO; School of Life Sciences, University of Nevada, Las Vegas, NV, United States., Mewalal R; US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, United States., Harmon-Smith M; US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, United States., Blaby IK; US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, United States., Cheng JF; US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, United States., Hess M; Department of Animal Science, College of Agricultural and Environmental Sciences, University of California, Davis, Davis, CA, United States., Brumm PJ; C5-6 Technologies LLC, Fitchburg, WI, United States., Singh NK; Biotechnology and Planetary Protection Group, Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA, United States., Venkateswaran K; Biotechnology and Planetary Protection Group, Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA, United States., Hedlund BP; School of Life Sciences, University of Nevada, Las Vegas, NV, United States.; Nevada Institute of Personalized Medicine, University of Nevada, Las Vegas, NV, United States. |
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| Jazyk: | angličtina |
| Zdroj: | Frontiers in microbiology [Front Microbiol] 2024 Apr 25; Vol. 15, pp. 1355444. Date of Electronic Publication: 2024 Apr 25 (Print Publication: 2024). |
| DOI: | 10.3389/fmicb.2024.1355444 |
| Abstrakt: | The aerobic hyperthermophile "Fervidibacter sacchari" catabolizes diverse polysaccharides and is the only cultivated member of the class "Fervidibacteria" within the phylum Armatimonadota . It encodes 117 putative glycoside hydrolases (GHs), including two from GH family 50 (GH50). In this study, we expressed, purified, and functionally characterized one of these GH50 enzymes, Fsa16295Glu. We show that Fsa16295Glu is a β-1,3-endoglucanase with optimal activity on carboxymethyl curdlan (CM-curdlan) and only weak agarase activity, despite most GH50 enzymes being described as β-agarases. The purified enzyme has a wide temperature range of 4-95°C (optimal 80°C), making it the first characterized hyperthermophilic representative of GH50. The enzyme is also active at a broad pH range of at least 5.5-11 (optimal 6.5-10). Fsa16295Glu possesses a relatively high k Competing Interests: PB was employed by C5-6 Technologies LLC. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The handling editor E-MZ declared a past collaboration with the author BH. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision. (Copyright © 2024 Covington, Torosian, Cook, Palmer, Bryan, Nou, Mewalal, Harmon-Smith, Blaby, Cheng, Hess, Brumm, Singh, Venkateswaran and Hedlund.) |
| Databáze: | MEDLINE |
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