| Autor: |
Teunissen MB, Kapsenberg ML, Stiekema FE, Keizer HG |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of immunological methods [J Immunol Methods] 1985 Feb 28; Vol. 77 (1), pp. 131-8. |
| DOI: |
10.1016/0022-1759(85)90190-5 |
| Abstrakt: |
The role of dendritic cells in antigen-induced murine T lymphocyte activation was studied by addition of purified dendritic cells to purified lymph node T lymphocytes from ovalbumin-primed mice. In the presence of the priming antigen T cells generated an antigen-specific response. The response was at least 3-fold higher with the use of a modified IMDM culture medium. The complete requirement for accessory cells was demonstrated only when nylon wool-purified T lymphocytes were thoroughly depleted of Ia antigen-expressing cells. Dendritic cells as well as peritoneal exudate macrophages were equally effective as antigen-presenting cells. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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