Blood Level of Nitric Oxide Metabolites and Expression of Genes Regulating NO Synthesis in Early Forms of Non-Alcoholic Fatty Liver Disease.

Autor: Kurbatova IV; Medical Biological Research Center, Karelian Research Centre, Russian Academy of Sciences, Petrozavodsk, Republic of Karelia, Russia. irina7m@yandex.ru.; Institute of Biology - Separated Subdivision of the Karelian Research Centre, Russian Academy of Sciences, Petrozavodsk, Republic of Karelia, Russia. irina7m@yandex.ru., Topchieva LV; Medical Biological Research Center, Karelian Research Centre, Russian Academy of Sciences, Petrozavodsk, Republic of Karelia, Russia.; Institute of Biology - Separated Subdivision of the Karelian Research Centre, Russian Academy of Sciences, Petrozavodsk, Republic of Karelia, Russia., Dudanova OP; Petrozavodsk State University, Petrozavodsk, Republic of Karelia, Russia., Shipovskaya AA; Petrozavodsk State University, Petrozavodsk, Republic of Karelia, Russia., Kantserova NP; Medical Biological Research Center, Karelian Research Centre, Russian Academy of Sciences, Petrozavodsk, Republic of Karelia, Russia.; Institute of Biology - Separated Subdivision of the Karelian Research Centre, Russian Academy of Sciences, Petrozavodsk, Republic of Karelia, Russia., Malysheva IE; Medical Biological Research Center, Karelian Research Centre, Russian Academy of Sciences, Petrozavodsk, Republic of Karelia, Russia.; Institute of Biology - Separated Subdivision of the Karelian Research Centre, Russian Academy of Sciences, Petrozavodsk, Republic of Karelia, Russia.
Jazyk: angličtina
Zdroj: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2024 Mar; Vol. 176 (5), pp. 555-561. Date of Electronic Publication: 2024 May 08.
DOI: 10.1007/s10517-024-06066-3
Abstrakt: The levels of NO metabolites in the plasma and mRNA of the NOS3, ATG9B, and NOS2 genes in peripheral blood leukocytes of healthy people and patients with early forms of non-alcoholic fatty liver disease (steatosis and weak activity non-alcoholic steatohepatitis) were studied. In patients with steatohepatitis, the concentration of NO metabolites in the blood and the level of mRNA of the NOS2 gene were higher than in patients with steatosis and healthy people. These differences can be of diagnostic value for distinguishing between steatosis and weak activity steatohepatitis in non-alcoholic fatty liver disease. A correlation between the levels of NO metabolites and the expression of the NOS2 gene in weak activity steatohepatitis was established, which indicates activation of NO synthesis in non-alcoholic steatohepatitis due to the expression of the inducible NO synthase gene. The level of the NOS2 gene mRNA in peripheral blood leukocytes of patients with weak activity steatohepatitis correlated with the level of TNFα and IL-6 cytokines. An increase in the level of NO in the blood in weak activity steatohepatitis correlated with the level of MDA, an indicator of oxidative stress.
(© 2024. Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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