| Autor: |
Holden CA; Lancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, UK., McAinsh MR; Lancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, UK., Taylor JE; Lancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, UK., Beckett P; Phlorum Ltd, Brighton BN2 6AH, UK., Albacete A; Institute for Agro-Environmental Research and Development of Murcia (IMIDA), Department of Plant Production and Agrotechnology, C/ Mayor s/n, La Alberca, E-30150 Murcia, Spain.; CEBAS-CSIC, Department of Plant Nutrition, Campus Universitario de Espinardo, E-30100 Murcia, Spain., Martínez-Andújar C; CEBAS-CSIC, Department of Plant Nutrition, Campus Universitario de Espinardo, E-30100 Murcia, Spain., Morais CLM; Center for Education, Science and Technology of the Inhamuns Region, State University of Ceará, Tauá 63660-000, Brazil.; Graduate Program in Chemistry, Institute of Chemistry, Federal University of Rio Grande do Norte, Natal 59072-970, Brazil., Martin FL; Department of Cellular Pathology, Blackpool Teaching Hospitals NHS Foundation Trust, Whinney Heys Road, Blackpool FY3 8NR, UK. francis.martin2@nhs.net.; Biocel UK Ltd, Hull HU10 6TS, UK. |
| Abstrakt: |
Plant hormones are important in the control of physiological and developmental processes including seed germination, senescence, flowering, stomatal aperture, and ultimately the overall growth and yield of plants. Many currently available methods to quantify such growth regulators quickly and accurately require extensive sample purification using complex analytic techniques. Herein we used ultra-performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS) to create and validate the prediction of hormone concentrations made using attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectral profiles of both freeze-dried ground leaf tissue and extracted xylem sap of Japanese knotweed ( Reynoutria japonica ) plants grown under different environmental conditions. In addition to these predictions made with partial least squares regression, further analysis of spectral data was performed using chemometric techniques, including principal component analysis, linear discriminant analysis, and support vector machines (SVM). Plants grown in different environments had sufficiently different biochemical profiles, including plant hormonal compounds, to allow successful differentiation by ATR-FTIR spectroscopy coupled with SVM. ATR-FTIR spectral biomarkers highlighted a range of biomolecules responsible for the differing spectral signatures between growth environments, such as triacylglycerol, proteins and amino acids, tannins, pectin, polysaccharides such as starch and cellulose, DNA and RNA. Using partial least squares regression, we show the potential for accurate prediction of plant hormone concentrations from ATR-FTIR spectral profiles, calibrated with hormonal data quantified by UHPLC-HRMS. The application of ATR-FTIR spectroscopy and chemometrics offers accurate prediction of hormone concentrations in plant samples, with advantages over existing approaches. |
