Changes in intrahost genetic diversity according to lesion severity in longitudinal HPV16 samples.

Autor: Costanzi JM; Department of Microbiology and Infection Control, Akershus University Hospital, Lørenskog, Norway.; Centre of Bioinformatics, Department of Pharmacy, University of Oslo, Oslo, Norway., Stosic MS; Department of Microbiology and Infection Control, Akershus University Hospital, Lørenskog, Norway.; Department of Life Sciences and Health, Faculty of Health Sciences, OsloMet-Oslo Metropolitan University, Oslo, Norway., Løvestad AH; Department of Microbiology and Infection Control, Akershus University Hospital, Lørenskog, Norway.; Department of Life Sciences and Health, Faculty of Health Sciences, OsloMet-Oslo Metropolitan University, Oslo, Norway.; Clinical Molecular Biology (EpiGen), Akershus University Hospital Lørenskog, Norway and Institute of Clinical Medicine, University of Oslo, Oslo, Norway., Ambur OH; Department of Life Sciences and Health, Faculty of Health Sciences, OsloMet-Oslo Metropolitan University, Oslo, Norway., Rounge TB; Centre of Bioinformatics, Department of Pharmacy, University of Oslo, Oslo, Norway.; Department of Research, Cancer Registry of Norway, Norwegian Institute of Public Health, Oslo, Norway., Christiansen IK; Department of Microbiology and Infection Control, Akershus University Hospital, Lørenskog, Norway.
Jazyk: angličtina
Zdroj: Journal of medical virology [J Med Virol] 2024 May; Vol. 96 (5), pp. e29641.
DOI: 10.1002/jmv.29641
Abstrakt: Human papillomavirus type 16 (HPV16) is the most common cause of cervical cancer, but most infections are transient with lesions not progressing to cancer. There is a lack of specific biomarkers for early cancer risk stratification. This study aimed to explore the intrahost HPV16 genomic variation in longitudinal samples from HPV16-infected women with different cervical lesion severity (normal, low-grade, and high-grade). The TaME-seq deep sequencing protocol was used to generate whole genome HPV16 sequences of 102 samples collected over time from 40 individuals. Single nucleotide variants (SNVs) and intrahost SNVs (iSNVs) were identified in the viral genomes. A majority of individuals had a unique set of SNVs and these SNVs were stable over time. Overall, the number of iSNVs and APOBEC3-induced iSNVs were significantly lower in high-grade relative to normal and low-grade samples. A significant increase in the number of APOBEC3-induced iSNVs over time was observed for normal samples when compared to high-grade. Our results indicates that the lower incidence of iSNVs and APOBEC3-induced iSNVs in high-grade lesions may have implications for novel biomarkers discoveries, potentially aiding early stratification of HPV-induced cervical precancerous lesions.
(© 2024 The Authors. Journal of Medical Virology published by Wiley Periodicals LLC.)
Databáze: MEDLINE
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