Leptin signaling promotes blood vessel formation in the Xenopus tail during the embryo-larval transition.

Autor: Curtis GH; School of Biological Sciences, Center for Reproductive Biology, Washington State University, Pullman, WA, USA, 99164. Electronic address: grace.curtis@wsu.edu., Reeve RE; School of Biological Sciences, Center for Reproductive Biology, Washington State University, Pullman, WA, USA, 99164., Crespi EJ; School of Biological Sciences, Center for Reproductive Biology, Washington State University, Pullman, WA, USA, 99164.
Jazyk: angličtina
Zdroj: Developmental biology [Dev Biol] 2024 Aug; Vol. 512, pp. 26-34. Date of Electronic Publication: 2024 May 03.
DOI: 10.1016/j.ydbio.2024.05.001
Abstrakt: The signals that regulate peripheral blood vessel formation during development are still under investigation. The hormone leptin promotes blood vessel formation, adipose tissue establishment and expansion, tumor growth, and wound healing, but the underlying mechanisms for these actions are currently unknown. We investigated whether leptin promotes angiogenesis in the developing tail fin using embryonic transgenic xflk-1:GFP Xenopus laevis, which express a green fluorescent protein on vascular endothelial cells to mark blood vessels. We found that leptin protein is expressed in endothelial cells of developing blood vessels and that leptin treatment via injection increased phosphorylated STAT3 signaling, which is indicative of leptin activation of its receptor, in blood vessels of the larval tail fin. Leptin administration via media increased vessel length, branching, and reconnection with the cardinal vein, while decreased leptin signaling via immunoneutralization had an opposing effect on vessel development. We also observed disorganization of major vessels and microvessels of the tail fin and muscle when leptin signaling was decreased. Reduced leptin signaling lowered mRNA expression of cenpk, gpx1, and mmp9, markers for cell proliferation, antioxidation, and extracellular matrix remodeling/cell migration, respectively, in the developing tail, providing insight into three possible mechanisms underlying leptin's promotion of angiogenesis. Together these results illustrate that leptin levels are correlated with embryonic angiogenesis and that leptin coordinates multiple aspects of blood vessel growth and development, showing that leptin is an important morphogen during embryonic development.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Published by Elsevier Inc.)
Databáze: MEDLINE
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