T-Cell Immune Responses in Newborns and Long-Term Sequelae in Congenital Cytomegalovirus Infection (CYTRIC Study).

Autor: Soriano-Ramos M; Centro de Salud Ciudad Jardín, Pediatric Department, Alicante, Spain. Electronic address: sorianoramosmaria@gmail.com., Pedrero-Tomé R; Instituto de Investigación Hospital 12 de Octubre (Imas12), Fundación Biomédica del Hospital Universitario 12 de Octubre (FBHU12O), Madrid, Spain., Giménez-Quiles E; Department of Microbiology, Microbiology Service, Hospital Clínico Universitario, INCLIVA Research Institute, Valencia, Spain., Albert E; Department of Microbiology, Microbiology Service, Hospital Clínico Universitario, INCLIVA Research Institute, Valencia, Spain., Baquero-Artigao F; Hospital Universitario Infantil La Paz, Department of Infectious Diseases and Tropical Pediatrics, Madrid, Spain; Centro de Investigación Biomédica en Red en Enfermedades Infecciosas (CIBERINFEC), Madrid, Spain., Rodríguez-Molino P; Hospital Universitario Infantil La Paz, Department of Infectious Diseases and Tropical Pediatrics, Madrid, Spain; Centro de Investigación Biomédica en Red en Enfermedades Infecciosas (CIBERINFEC), Madrid, Spain., Del Rosal T; Hospital Universitario Infantil La Paz, Department of Infectious Diseases and Tropical Pediatrics, Madrid, Spain; Centro de Investigación Biomédica en Red en Enfermedades Raras (CIBERER), Madrid, Spain., Noguera-Julian A; Department of Pediatric Infectious Diseases, Malalties Infeccioses I Resposta Inflamatòria Sistèmica en Pediatria, Servei de Malalties Infeccioses I Patologia Importada, Institut de Recerca Pediàtrica Sant Joan de Déu, Barcelona, Spain; Departament de Cirurgia i Especialitats Medicoquirúrgiques, Facultat de Medicina i Ciències de la Salut, Universitat de Barcelona, Barcelona, Spain; Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública, Instituto de Salud Carlos III, Madrid, Spain., Fortuny C; Department of Pediatric Infectious Diseases, Malalties Infeccioses I Resposta Inflamatòria Sistèmica en Pediatria, Servei de Malalties Infeccioses I Patologia Importada, Institut de Recerca Pediàtrica Sant Joan de Déu, Barcelona, Spain; Departament de Cirurgia i Especialitats Medicoquirúrgiques, Facultat de Medicina i Ciències de la Salut, Universitat de Barcelona, Barcelona, Spain; Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública, Instituto de Salud Carlos III, Madrid, Spain., Ríos-Barnés M; Department of Pediatric Infectious Diseases, Malalties Infeccioses I Resposta Inflamatòria Sistèmica en Pediatria, Servei de Malalties Infeccioses I Patologia Importada, Institut de Recerca Pediàtrica Sant Joan de Déu, Barcelona, Spain., Saavedra-Lozano J; Department of Pediatric Infectious Diseases, Hospital General Universitario Gregorio Marañón, Pediatric Infectious Diseases Unit, Universidad Complutense, Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañón (IiSGM), CIBER Enfermedades Infecciosas (CIBERINFEC), Madrid, Spain., Dueñas E; Department of Pediatric Infectious Diseases, Hospital General Universitario Gregorio Marañón, Pediatric Infectious Diseases Unit, Universidad Complutense, Madrid, Spain., Sánchez-Mateos M; Department of Neonatology, Hospital Universitario Puerta de Hierro, Madrid, Spain., Castells L; Department of Neonatology, Pediatric Unit, Hospital Quirónsalud del Vallès, Barcelona, Spain., de la Serna M; Department of Neonatology, Hospital Infanta Sofía, San Sebastián de los Reyes, Madrid, Spain., Frick MA; Pediatric Infectious Diseases Unit, Department of Pediatric Infectious Diseases, Hospital Vall d'Hebron, Barcelona, Spain., de Vergas J; Hospital Universitario 12 de Octubre, Pediatric Otorhinolaryngology Department, Madrid, Spain., Núñez-Enamorado N; Pediatric Neurology Department, Hospital Universitario 12 de Octubre, Madrid, Spain., Moral-Pumarega MT; Department of Neonatology, Hospital Universitario 12 de Octubre, Madrid, Spain., Folgueira MD; Microbiology Department, Hospital Universitario 12 de Octubre, Madrid, Spain., Navarro D; Department of Microbiology, Microbiology Service, Hospital Clínico Universitario, INCLIVA Research Institute, Valencia, Spain; Department of Microbiology, School of Medicine, University of Valencia, Spain; Centro de Investigación Biomédica en Red de Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III, Madrid, Spain., Blázquez-Gamero D; Instituto de Investigación Hospital 12 de Octubre (Imas12), Fundación Biomédica del Hospital Universitario 12 de Octubre (FBHU12O), Madrid, Spain; Pediatric Infectious Diseases Unit, Department of Pediatric Infectious Diseases, Hospital Universitario 12 de Octubre, RITIP, Madrid, Spain; Universidad Complutense de Madrid, Madrid, Spain.
Jazyk: angličtina
Zdroj: The Journal of pediatrics [J Pediatr] 2024 Sep; Vol. 272, pp. 114084. Date of Electronic Publication: 2024 May 04.
DOI: 10.1016/j.jpeds.2024.114084
Abstrakt: Objective: The objective of this study was to assess the role of T-lymphocyte immune responses in newborns with congenital cytomegalovirus (CMV) infection (cCMV) and their potential association with the development of long-term sequelae.
Study Design: A multicenter, prospective study from 2017 to 2022 was conducted across 8 hospitals in Spain. Blood samples were collected within the first month of life from neonates diagnosed with cCMV. Intracellular cytokine staining was employed to evaluate the presence of CMV-specific interferon-gamma (IFN-γ)-producing CD8 + and CD4 + T lymphocytes (CMV-IFN-γ-CD8 + /CD4 + ) using flow cytometry. The development of sequelae, including hearing loss and neurologic impairment, was assessed during follow-up.
Results: In total, 64 newborns were included; 42 infants (65.6%) had symptomatic cCMV. The median age at the last follow-up visit was 25.3 months (IQR 20.1-34.4). Eighteen infants had long-term sequelae (28.1%), predominantly hearing loss (20.3%) and neurologic disorders (15.6%). No relationship was observed between total count or percentage of CMV-specific IFN-γ-CD8 + or CD4 + lymphocytes and long-term sequelae. Multivariable analysis demonstrated an association between lower total lymphocyte count and long-term sequelae (aOR 0.549, 95% CI: 0.323-0.833), which requires further study.
Conclusions: CMV-specific IFN-γ-CD4 + and CD8 + T-lymphocyte responses in neonates with cCMV were not predictive of long-term sequelae.
Competing Interests: Declaration of Competing Interest DBG received fees from MSD as speaker in educational activities. JSL received fees from Biomerieux as speaker. None of the remaining authors have any conflict of interests to declare. This work was supported by projects PI 16/00807, PI 19/01333, and PI 22/01540, from the Instituto de Salud Carlos III (Ministry of Economy, Industry and Competitiveness) and co-funded by the European Regional Development Fund. DBG was supported by the Spanish Ministry of Science and Innovation - Instituto de Salud Carlos III and by Fondos FEDER “Contratos para la intensificación de la actividad investigadora en el Sistema Nacional de Salud, 2020 INT20/00  086 and 2023 INT23/00  039”. The study was approved by the Ethics Committee of Hospital Universitario 12 de Octubre. Paula Rodríguez-Molino was funded by the Spanish Ministry of Science and Innovation-Instituto de Salud Carlos III and Fondos FEDER (Contrato Río Hortega CM21/00  174).
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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