Chitosan-Coated Fosetyl-Al Nanocrystals' Efficacy on Nicotiana tabacum Colonized by Xylella fastidiosa .

Autor:	Tatulli G; Council for Agricultural Research and Economics, Research Centre for Plant Protection and Certification of Rome, 00156 Rome, Italy., Baldassarre F; Department of Biological and Environmental Sciences, UdR INSTM of Lecce University of Salento, Via Monteroni, 73100 Lecce, Italy.; Institute of Nanotechnology, CNR NANOTEC, Consiglio Nazionale delle Ricerche, Via Monteroni, 73100 Lecce, Italy., Schiavi D; Department of Agriculture and Forest Sciences (DAFNE), University of Tuscia, Via S. Camillo de Lellis, Snc, 01100 Viterbo, Italy., Tacconi S; CarMeN Laboratory, INSERM 1060-INRAE 1397, Department of Human Nutrition, Lyon Sud Hospital, University of Lyon, Lyon, France., Cognigni F; Department of Basic and Applied Sciences for Engineering (SBAI), Sapienza University of Rome, Rome, Italy., Costantini F; Council for Agricultural Research and Economics, Research Centre for Plant Protection and Certification of Rome, 00156 Rome, Italy.; Department of Environmental Biology, Sapienza University of Rome, p.le A. Moro 5, 00185, Rome, Italy., Balestra GM; Department of Agriculture and Forest Sciences (DAFNE), University of Tuscia, Via S. Camillo de Lellis, Snc, 01100 Viterbo, Italy.; Phytoparasites Diagnostics (PhyDia) s.r.l. Via S. Camillo Delellis Snc 01100 Viterbo, Italy., Dini L; Department of Biology and Biotechnology 'C. Darwin', Sapienza University of Rome, Rome, Italy., Pucci N; Council for Agricultural Research and Economics, Research Centre for Plant Protection and Certification of Rome, 00156 Rome, Italy., Rossi M; Department of Basic and Applied Sciences for Engineering (SBAI), Sapienza University of Rome, Rome, Italy.; Research Center on Nanotechnology Applied to Engineering of Sapienza (CNIS), Sapienza University of Rome, Rome, Italy., Scala V; Council for Agricultural Research and Economics, Research Centre for Plant Protection and Certification of Rome, 00156 Rome, Italy., Ciccarella G; Department of Biological and Environmental Sciences, UdR INSTM of Lecce University of Salento, Via Monteroni, 73100 Lecce, Italy.; Institute of Nanotechnology, CNR NANOTEC, Consiglio Nazionale delle Ricerche, Via Monteroni, 73100 Lecce, Italy., Loreti S; Council for Agricultural Research and Economics, Research Centre for Plant Protection and Certification of Rome, 00156 Rome, Italy.
Jazyk:	angličtina
Zdroj:	Phytopathology [Phytopathology] 2024 Jul; Vol. 114 (7), pp. 1466-1479. Date of Electronic Publication: 2024 Jul 11.
DOI:	10.1094/PHYTO-04-24-0144-R
Abstrakt:	Xylella fastidiosa ( Xf ) is a quarantine plant pathogen capable of colonizing the xylem of a wide range of hosts. Currently, there is no cure able to eliminate the pathogen from a diseased plant, but several integrated strategies have been implemented for containing the spread of Xf . Nanotechnology represents an innovative strategy based on the possibility of maximizing the potential antibacterial activity by increasing the surface-to-volume ratio of nanoscale formulations. Nanoparticles based on chitosan and/or fosetyl-Al have shown different in vitro antibacterial efficacy against Xf subsp. fastidiosa ( Xff ) and pauca ( Xfp ). This work demonstrated the uptake of chitosan-coated fosetyl-Al nanocrystals (CH-nanoFos) by roots and their localization in the stems and leaves of Olea europaea plants. Additionally, the antibacterial activity of fosetyl-Al, nano-fosetyl, nano-chitosan, and CH-nanoFos was tested on Nicotiana tabacum cultivar SR1 (Petite Havana) inoculated with Xff, Xfp , or Xf subsp. multiplex ( Xfm ). The bacterial load was evaluated with qPCR, and the results showed that CH-nanoFos was the only treatment able to reduce the colonization of Xff, Xfm , and Xfp in tobacco plants. Additionally, the area under the disease progress curve, used to assess symptom development in tobacco plants inoculated with Xff, Xfm , and Xfp and treated with CH-nanoFos, showed a reduction in symptom development. Furthermore, the twitching assay and bacterial growth under microfluidic conditions confirmed the antibacterial activity of CH-nanoFos. Competing Interests: The author(s) declare no conflict of interest.
Databáze:	MEDLINE
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