The role of CD20 + T cells: Insights in human peripheral blood.

Autor:	Pinho ACO; CNC-UC- Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.; CIBB -Center for Innovative Biomedicine and Biotechnology, University of Coimbra, Coimbra, Portugal.; Department of Life Sciences, Faculty of Science and Technology, University of Coimbra, Coimbra, Portugal., Barbosa P; CNC-UC- Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.; CIBB -Center for Innovative Biomedicine and Biotechnology, University of Coimbra, Coimbra, Portugal.; Institute for Interdisciplinary Research, University of Coimbra, Coimbra, Portugal., Pereira MJ; Department of Medical Sciences, Clinical Diabetology and Metabolism, Uppsala University, Uppsala, Sweden., Paiva A; CIBB -Center for Innovative Biomedicine and Biotechnology, University of Coimbra, Coimbra, Portugal.; Flow Cytometry Unit, Clinical Pathology Department, Hospitais da Universidade de Coimbra, Unidade Local de Saúde de Coimbra, Coimbra, Portugal.; Coimbra Institute for Clinical and Biomedical Research (iCBR), Group of Environmental Genetics of Onco-biology (CIMAGO), Faculty of Medicine (FMUC), University of Coimbra, Coimbra, Portugal.; Clinical Academic Center of Coimbra (CACC), Coimbra, Portugal.; Instituto Politécnico de Coimbra, ESTESC-Coimbra Health School, Ciências Biomédicas Laboratoriais, Coimbra, Portugal., Carvalho E; CNC-UC- Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.; CIBB -Center for Innovative Biomedicine and Biotechnology, University of Coimbra, Coimbra, Portugal.; Institute for Interdisciplinary Research, University of Coimbra, Coimbra, Portugal.; APDP-Portuguese Diabetes Association, Lisbon, Portugal., Laranjeira P; CNC-UC- Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.; CIBB -Center for Innovative Biomedicine and Biotechnology, University of Coimbra, Coimbra, Portugal.; Flow Cytometry Unit, Clinical Pathology Department, Hospitais da Universidade de Coimbra, Unidade Local de Saúde de Coimbra, Coimbra, Portugal.; Coimbra Institute for Clinical and Biomedical Research (iCBR), Group of Environmental Genetics of Onco-biology (CIMAGO), Faculty of Medicine (FMUC), University of Coimbra, Coimbra, Portugal.; Clinical Academic Center of Coimbra (CACC), Coimbra, Portugal.
Jazyk:	angličtina
Zdroj:	Cytometry. Part B, Clinical cytometry [Cytometry B Clin Cytom] 2024 May; Vol. 106 (3), pp. 171-180. Date of Electronic Publication: 2024 May 02.
DOI:	10.1002/cyto.b.22178
Abstrakt:	CD20 + T cells constitute a small subset of T cells. These are found among CD4 + , CD8 + , CD4 + CD8 + , CD4 - CD8 - T, and TCRγδ + T cells, and have been poorly characterized. The aim of this study was to characterize peripheral blood (PB) CD20 + T cells and compare them to their PB CD20 - T cell counterparts. PB from 17 healthy individuals was collected. The distribution of CD20 + T cells among maturation-associated T cells compartments (naïve, central memory, transitional memory, effector memory, and effector T cells), their polarization, activation status, and expression of immune-regulatory proteins were evaluated by flow cytometry. Their function was also assessed, by measuring IFN-γ, TNF-α, and IL-17 production. Compared with CD20 - T cells, CD20 + T cells represent a higher proportion of transitional memory cells. Furthermore, CD20 + T cells display a proinflammatory phenotype, characterized by the expansion of Th1, Th1/17, and Tc1 cell subsets , associated to a high expression of activation (CD25) and exhaustion (PD-1) markers. In addition, the simultaneous production of the proinflammatory cytokines IFN-γ, TNF-α, and IL-17 was also detected in CD4 + CD20 + T cells. Our results show that CD20 + T cells are phenotypically and functionally different from CD20 - T cells, suggesting that these cells are a distinct subset of T cells. (© 2024 The Authors. Cytometry Part B: Clinical Cytometry published by Wiley Periodicals LLC on behalf of International Clinical Cytometry Society.)
Databáze:	MEDLINE
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