Association Between Muscle Growth and Transcription of a Mutant MSTN Gene in Olive Flounder (Paralichthys olivaceus).

Autor: Kim JW; Biotechnology Research Division, National Institute of Fisheries Science, Busan, 46083, Republic of Korea., Kim J; Genetics and Breeding Research Center, National Institute of Fisheries Science, Geoje, 53334, Republic of Korea., Cho JY; Biotechnology Research Division, National Institute of Fisheries Science, Busan, 46083, Republic of Korea., Shin Y; Research and Development Center, Insilicogen Inc., Yongin-si, 16954, Republic of Korea., Son H; Research and Development Center, Insilicogen Inc., Yongin-si, 16954, Republic of Korea., Sathiyamoorthy S; Research and Development Center, Insilicogen Inc., Yongin-si, 16954, Republic of Korea., Kim BS; Department of Aquatic Life Medicine, Kunsan National University, Gunsan, 54150, Republic of Korea., Kim YO; Biotechnology Research Division, National Institute of Fisheries Science, Busan, 46083, Republic of Korea., Kang BC; D.iF Inc., Yongin-si, 16954, Republic of Korea. bckang@d-if.kr., Kong HJ; Biotechnology Research Division, National Institute of Fisheries Science, Busan, 46083, Republic of Korea. heejkong@korea.kr.
Jazyk: angličtina
Zdroj: Marine biotechnology (New York, N.Y.) [Mar Biotechnol (NY)] 2024 Jun; Vol. 26 (3), pp. 599-608. Date of Electronic Publication: 2024 Apr 29.
DOI: 10.1007/s10126-024-10322-y
Abstrakt: Myostatin (MSTN, also known as growth differentiation factor-8 (GDF-8)), a member of the transforming growth factor β (TGF-β) superfamily, functions as a negative regulator of skeletal muscle development and growth. However, it is also expressed in a wide range of tissues in fish and thus may have more diverse roles in this group than in mammals. In this study, we assessed the genome-wide transcriptional expression pattern associated with the CRISPR/Cas9-mutated MSTN gene in the olive flounder (Paralichthys olivaceus) in association with changes in cell proliferation and transportation processes. There were no differences in the hepatosomatic index, and the growth of male and female fish increased in the F1 progeny of the MSTN mutants. Furthermore, the histopathological analysis showed that myostatin editing resulted in a 41.24% increase in back muscle growth and 46.92% increase in belly muscle growth in male flounder compared with normal flounder, and a 16.01% increase in back muscle growth and 14.26% increase in belly muscle growth in female flounder compared with normal flounder. This study demonstrates that editing of the myostatin gene enhances muscle growth in olive flounder, with a notably more pronounced effect observed in males. Consequently, myostatin-edited male flounder could represent a valuable asset for the flounder aquaculture industry.
(© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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