Antibodies anti-rFilF protein has anti-biofilm activity against carbapenem-resistant Acinetobacter baumannii.

Autor: Pereira IL; Biotechnology Unit, Center of Technological Development, Federal University of Pelotas, CEP 96010-900, Pelotas, RS, Brazil., Cardoso TL; Biotechnology Unit, Center of Technological Development, Federal University of Pelotas, CEP 96010-900, Pelotas, RS, Brazil., Wozeak DR; Biotechnology Unit, Center of Technological Development, Federal University of Pelotas, CEP 96010-900, Pelotas, RS, Brazil., Caballero PS; Biotechnology Unit, Center of Technological Development, Federal University of Pelotas, CEP 96010-900, Pelotas, RS, Brazil., Buchhorn de Freitas S; Biotechnology Unit, Center of Technological Development, Federal University of Pelotas, CEP 96010-900, Pelotas, RS, Brazil., Pinto Seixas Neto AC; Department of Microbiology and Parasitology, Biology Institute, Federal University of Pelotas, CEP 96010-900, Pelotas, RS, Brazil., da Silva Pinto L; Biotechnology Unit, Center of Technological Development, Federal University of Pelotas, CEP 96010-900, Pelotas, RS, Brazil., Hartwig DD; Biotechnology Unit, Center of Technological Development, Federal University of Pelotas, CEP 96010-900, Pelotas, RS, Brazil; Department of Microbiology and Parasitology, Biology Institute, Federal University of Pelotas, CEP 96010-900, Pelotas, RS, Brazil. Electronic address: daianehartwig@gmail.com.
Jazyk: angličtina
Zdroj: Microbes and infection [Microbes Infect] 2024 Jul-Aug; Vol. 26 (5-6), pp. 105347. Date of Electronic Publication: 2024 Apr 27.
DOI: 10.1016/j.micinf.2024.105347
Abstrakt: Acinetobacter baumannii is an opportunistic bacterium that causes infection in several sites. Carbapenem-resistant A. baumannii strains (CRAb) lead the World Health Organization's list of 12 pathogens considered a priority for developing new antimicrobials. The pathogenicity of A. baumannii is related to the different virulence factors employed in the colonization of biotic and abiotic surfaces, biofilm formation and multidrug resistance. We analyze the outer membrane protein FilF from A. baumannii in silico and produce it in recombinant form (rFilF). rFilF protein was successfully expressed in Escherichia coli BL21 Star in an insoluble form. Immunization with rFilF induced significant anti-rFilF IgG antibody production in mice, detected by indirect enzyme-linked immunosorbent assay, since the first evaluation until 49th. On the last experimentation day, the predominant immunoglobulin found was IgG1 followed by IgG2a, IgG2b, IgM, IgG3, and IgA. We observe that interleukins 4 and 10 show significant production after the 28th day of experimentation in mice immunized with rFilF. Anti-rFilF pAbs were able to inhibit biofilm formation in nine CRAb strains evaluated, and in the standard strain ATCC® 19606. These results demonstrate the anti-biofilm activity of anti-rFilF antibodies, promising in the development of a non-antibiotic approach based on the control of CRAb strains.
Competing Interests: Declaration of competing interest The authors declare no conflict of interest. The founding sponsors had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, and in the decision to publish the results.
(Copyright © 2024 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.)
Databáze: MEDLINE
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