Long-Term Treatment with Alcaligenes faecalis A12C Improves Host Resistance to Pathogens in Septic Rats: Possible Contribution of Curdlan-Like Immune Trainer.

Autor: Martel-Benítez CJ; Group of Fish health for aquaculture and wild species, Infectious diseases and Food safety, University Institute of Animal Health and Food Safety (IUSA), University of Las Palmas de Gran Canaria, 35416, Arucas, Spain., Alayón-Afonso R; Group of Fish health for aquaculture and wild species, Infectious diseases and Food safety, University Institute of Animal Health and Food Safety (IUSA), University of Las Palmas de Gran Canaria, 35416, Arucas, Spain., Castillo DP; Group of Fish health for aquaculture and wild species, Infectious diseases and Food safety, University Institute of Animal Health and Food Safety (IUSA), University of Las Palmas de Gran Canaria, 35416, Arucas, Spain. daniel.padilla@ulpgc.es., Chamizo-López FJ; Microbiology Department, Hospital Universitario de Gran Canaria Dr. Negrín, Barranco de La Ballena s/n, 35019, Las Palmas de Gran Canaria, Spain., García-Laorden MI; CIBER de Enfermedades Respiratorias, Instituto de Salud Carlos III, Monforte de Lemos 3-5, Pabellón 11, 28029, Madrid, Spain.; Multidisciplinary Organ Dysfunction Evaluation Research Network, Research Unit, Hospital Universitario de Gran Canaria Dr. Negrín, Barranco de La Ballena s/n, 35019, Las Palmas de Gran Canaria, Spain., de Los Monteros Y Zayas AE; Morphology Department, University Institute of Animal Health and Food Safety (IUSA), Universidad de Las Palmas de Gran Canaria, Arucas, Las Palmas, Spain., Rivero-Vera JC; Pathology Service, Hospital Universitario de Gran Canaria Dr. Negrín, Barranco de La Ballena S/N, 35019, Las Palmas de Gran Canaria, Spain., Salgueiro PN; Clinical Biochemistry Department, Hospital Universitario de Gran Canaria Dr. Negrín, Barranco de La Ballena s/n, 35019, Las Palmas de Gran Canaria, Spain., Real F; Group of Fish health for aquaculture and wild species, Infectious diseases and Food safety, University Institute of Animal Health and Food Safety (IUSA), University of Las Palmas de Gran Canaria, 35416, Arucas, Spain., Bordes-Benítez A; Microbiology Department, Hospital Universitario de Gran Canaria Dr. Negrín, Barranco de La Ballena s/n, 35019, Las Palmas de Gran Canaria, Spain., Quintana AM; Banco Español de Algas, Instituto de Oceanografía y Cambio Global, Universidad de Las Palmas de Gran Canaria, Telde, Spain., Peña CA; Banco Español de Algas, Fundación Parque Científico Tecnológico, Universidad de Las Palmas de Gran Canaria, Telde, Spain., Cabrera CD; Clinical Biochemistry Department, Hospital Universitario de Gran Canaria Dr. Negrín, Barranco de La Ballena s/n, 35019, Las Palmas de Gran Canaria, Spain., González-Martín JM; Statistics Service, Research Unit, Hospital Universitario de Gran Canaria Dr. Negrín, Barranco de La Ballena s/n, 35019, Las Palmas de Gran Canaria, Spain., Caballero JM; Barcelona, Biomedical Research Park (PRBB), Barcelona, Spain., Beneyto RF; Deparment of Laboratory Medicine and Comparative Medicine, Karolinska Institutet, Stockholm, Sweden., Villar J; CIBER de Enfermedades Respiratorias, Instituto de Salud Carlos III, Monforte de Lemos 3-5, Pabellón 11, 28029, Madrid, Spain.; Multidisciplinary Organ Dysfunction Evaluation Research Network, Research Unit, Hospital Universitario de Gran Canaria Dr. Negrín, Barranco de La Ballena s/n, 35019, Las Palmas de Gran Canaria, Spain.; Fundación Canaria del Instituto de Investigación Sanitaria de Canarias (FIISC), Las Palmas de Gran Canaria, Spain., Martín-Barrasa JL; Group of Fish health for aquaculture and wild species, Infectious diseases and Food safety, University Institute of Animal Health and Food Safety (IUSA), University of Las Palmas de Gran Canaria, 35416, Arucas, Spain.; Fundación Canaria del Instituto de Investigación Sanitaria de Canarias (FIISC), Las Palmas de Gran Canaria, Spain.; Animal Facility, Research Unit, Hospital Universitario de Gran Canaria Dr. Negrín, Barranco de La Ballena s/n, 35019, Las Palmas de Gran Canaria, Spain.; CIBER de Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III, Madrid, Spain.
Jazyk: angličtina
Zdroj: Probiotics and antimicrobial proteins [Probiotics Antimicrob Proteins] 2024 Apr 26. Date of Electronic Publication: 2024 Apr 26.
DOI: 10.1007/s12602-024-10252-0
Abstrakt: Curdlan, a β-1,3/1,6-glucan found in Alcaligenes faecalis (A. faecalis) wall, activates innate and humoral immunity. The aim of this study is to evaluate whether pretreated rats with A. faecalis A12C could prevent sepsis disturbances and identify the immunomodulatory mechanisms involved. Experiments occurred in two stages: a survival study with 16 rats randomly divided into septic (SC) (n = 8) and septic pretreated (SA) (n = 8) groups and 45 rats divided into four groups: healthy (AGUSAN) (n = 9), septic (AGUIC) (n = 13), septic pretreated (AGUIA) (n = 14), and healthy pretreated (AGUSTO) (n = 9). Sepsis was induced by cecal ligation and puncture after 30 days of A. faecalis A12C pretreatment or without. SA group had a higher survival rate of 58% vs. 16% for SC group (P < 0.05). Overall, AGUIA showed better status than AGUIC (P < 0.01). Higher monocytosis was found in AGUIA and AGUSTO vs. AGUIC and AGUSAN, respectively (P < 0.05). A gradual increase in curdlan fecal concentration was observed in AGUIA during pretreatment. Fecal concentrations of Escherichia coli significantly decreased in AGUIA and AGUSTO. Bacterial load in urine, peritoneal lavage fluid (PLF), and bronchoalveolar lavage fluid (BALF) decreased (P < 0.05) in AGUIA vs. AGUIC. Finally, lower inflammation was observed in serum, BALF, and PLF, with reduced IL-6, IL-10, IL-1β, and TNF-α, along with less damage in lungs and peritoneum in AGUIA vs. AGUIC. These findings suggest the connection between curdlan-produced by A. faecalis A12C-with the immune system and the reduction in severity of experimental sepsis.
(© 2024. The Author(s).)
Databáze: MEDLINE
Externí odkaz: