[Reversal Effect of NVP-BEZ235 on Doxorubicin-Resistance in Burkitt Lymphoma RAJI Cell Line].
| Autor: | Li CT; Department of Hematology， The First Hospital of Quanzhou Affiliated to Fujian Medical Uinversity, Quanzhou 362200, Fujian Province, China E-mail: lct090909@163.com., Zhu XP; Department of Hematology，The First Hospital of Quanzhou Affiliated to Fujian Medical Uinversity, Quanzhou 362200, Fujian Province, China., Wang SX; Department of Hematology，The First Hospital of Quanzhou Affiliated to Fujian Medical Uinversity, Quanzhou 362200, Fujian Province, China., Peng QY; Department of Hematology，The First Hospital of Quanzhou Affiliated to Fujian Medical Uinversity, Quanzhou 362200, Fujian Province, China., Zheng Y; Department of Hematology，The First Hospital of Quanzhou Affiliated to Fujian Medical Uinversity, Quanzhou 362200, Fujian Province, China., Liu SQ; Department of Hematology，The First Hospital of Quanzhou Affiliated to Fujian Medical Uinversity, Quanzhou 362200, Fujian Province, China., Lu XD; Department of Clinical Medicine, Fujian Medical University， Fuzhou 350004, Fujian Province, China., Wang YS; Department of Clinical Medicine, Fujian Medical University， Fuzhou 350004, Fujian Province, China., Weng D; Department of Clinical Medicine, Fujian Medical University， Fuzhou 350004, Fujian Province, China., Wang D; Department of Clinical Medicine, Fujian Medical University， Fuzhou 350004, Fujian Province, China. |
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| Jazyk: | čínština |
| Zdroj: | Zhongguo shi yan xue ye xue za zhi [Zhongguo Shi Yan Xue Ye Xue Za Zhi] 2024 Apr; Vol. 32 (2), pp. 476-482. |
| DOI: | 10.19746/j.cnki.issn.1009-2137.2024.02.024 |
| Abstrakt: | Objective: To study the reversal effect of NVP-BEZ235 on doxorubicin resistance in Burkitt lymphoma RAJI cell line. Methods: The doxorubicin-resistant cell line was induced by treating RAJI cells with a concentration gradient of doxorubicin. The levels of Pgp, p-AKT, and p-mTOR in cells were detected by Western blot. Cell viability was detected by MTT assay. IC Results: The doxorubicin-resistant Burkitt lymphoma cell line, RAJI/DOX, was established successfully. The expression of Pgp and the phosphorylation levels of AKT and mTOR in RAJI/DOX cell line were both higher than those in RAJI cell line. NVP-BEZ235 downregulated the phosphorylation levels of AKT and mTOR in RAJI/DOX cell line. NVP-BEZ235 inhibited the proliferation of RAJI/DOX cell line, and the effect was obvious when it was cooperated with doxorubicin. Conclusion: The constitutive activation of PI3K/AKT/mTOR pathway of RAJI/DOX cell line was more serious than RAJI cell line. NVP-BEZ235 reversed doxorubicin resistance of RAJI/DOX cell line by inhibiting the PI3K/AKT/mTOR signal pathway. |
| Databáze: | MEDLINE |
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