Diverse Class 2 CRISPR Effectors as Active Nucleases with Expanded Targeting Capabilities.
| Autor: | Wang M; UCB Biosciences Inc, Early Solutions, Cambridge, Massachusetts, USA., Rieber L; UCB Biosciences Inc, Early Solutions, Durham, North Carolina, USA., van Baaren J; UCB Biosciences Inc, Early Solutions, Cambridge, Massachusetts, USA., Morgan M; UCB Biosciences Inc, Early Solutions, Durham, North Carolina, USA., Merrett S; UCB Biosciences Inc, Early Solutions, Durham, North Carolina, USA., McDowell I; UCB Biosciences Inc, Early Solutions, Durham, North Carolina, USA., Bowen T; UCB Biosciences Inc, Early Solutions, Durham, North Carolina, USA. |
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| Jazyk: | angličtina |
| Zdroj: | The CRISPR journal [CRISPR J] 2024 Apr; Vol. 7 (2), pp. 120-130. |
| DOI: | 10.1089/crispr.2023.0058 |
| Abstrakt: | CRISPR-Cas systems have proven effective in a variety of applications due to their ease of use and relatively high editing efficiency. Yet, any individual CRISPR-Cas system has inherent limitations, necessitating a diversity of RNA-guided nucleases to suit applications with distinct needs. We searched through metagenomic sequences to identify RNA-guided nucleases and found enzymes from diverse CRISPR-Cas types and subtypes, the most promising of which we developed into gene-editing platforms. Based on prior annotations of the metagenomic sequences, we establish the likely taxa and sampling locations where Class 2 CRISPR-Cas systems active in eukaryotes may be found. The newly discovered systems show robust capabilities as gene editors and base editors. |
| Databáze: | MEDLINE |
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