Impact of rumen cannulation surgery on rumen microbiota composition in Hanwoo steers.

Autor:	Kim M; Division of Animal Science, Chonnam National University, Gwangju 61186, Korea., Park T; Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Korea., Park C; Division of Animal Science, Chonnam National University, Gwangju 61186, Korea., Baek YC; Animal Nutrition & Physiology Division, National Institute of Animal Science, Wanju 55365, Korea., Cho A; Division of Animal Diseases & Health, National Institute of Animal Science, Wanju 55365, Korea., Lee HG; Division of Animal Diseases & Health, National Institute of Animal Science, Wanju 55365, Korea., Kim E; Division of Animal Diseases & Health, National Institute of Animal Science, Wanju 55365, Korea., Bok EY; Division of Animal Diseases & Health, National Institute of Animal Science, Wanju 55365, Korea., Jung YH; Division of Animal Diseases & Health, National Institute of Animal Science, Wanju 55365, Korea., Hur TY; Division of Animal Diseases & Health, National Institute of Animal Science, Wanju 55365, Korea., Do YJ; Division of Animal Diseases & Health, National Institute of Animal Science, Wanju 55365, Korea.
Jazyk:	angličtina
Zdroj:	Journal of animal science and technology [J Anim Sci Technol] 2024 Mar; Vol. 66 (2), pp. 353-365. Date of Electronic Publication: 2024 Mar 31.
DOI:	10.5187/jast.2024.e17
Abstrakt:	Rumen cannulation is a surgical technique used to collect rumen contents from ruminants. However, rumen cannulation surgery may potentially impact the composition of the rumen microbiota. This study aimed to examine the longitudinal alterations in the rumen microbiota composition of Hanwoo steers after cannulation surgery. In this study, eight Hanwoo steers were used; four steers underwent rumen cannulation surgery (cannulation group), while the remaining four were left intact (control group). Rumen samples were collected from all eight steers using the stomach tubing method on the day before surgery (day 0) and on postoperative days 1, 4, 7, 10, 14, 17, 21, 24, and 28, resulting in 80 samples (10 timepoints × 8 animals). The microbiota of all 80 samples were analyzed using 16S rRNA gene amplicon sequencing with Quantitative Insights into Microbial Ecology version 2 (QIIME2). There were no significant differences ( p > 0.05) in all major phyla and most major genera representing at least 0.5% of total sequences across all 80 samples between the control and cannulation groups on the preoperative and postoperative days. However, while the alpha diversity indices did not differ ( p > 0.05) between the two groups on the preoperative day, they significantly differed ( p < 0.05) between the two groups on the postoperative days. Further, the overall microbial distribution based on both unweighted and weighted principal coordinate analysis plots significantly differed ( p < 0.05) between the two groups on both the preoperative and postoperative days. Orthogonal polynomial contrasts indicated that major genera and microbial diversity in the cannulation group decreased following surgery but returned to their initial states by postoperative day 28. In conclusion, this study demonstrates that rumen cannulation surgery affects some major taxa and microbial diversity, suggesting that the rumen cannulation method can alter the composition of rumen microbiota in Hanwoo steers. Competing Interests: No potential conflict of interest relevant to this article was reported. (© Copyright 2024 Korean Society of Animal Science and Technology.)
Databáze:	MEDLINE
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