Ancient origin of the rod bipolar cell pathway in the vertebrate retina.

Autor: Hellevik AM; Department of Biological Structure, University of Washington, Seattle, WA, USA., Mardoum P; Department of Biological Structure, University of Washington, Seattle, WA, USA., Hahn J; Department of Chemical and Biomolecular Engineering; Helen Wills Neuroscience Institute; Vision Sciences Graduate Program; California Institute of Quantitative Biosciences (QB3), University of California Berkley, Berkeley, CA, USA., Kölsch Y; Department Genes - Circuits - Behavior, Max Planck Institute for Biological Intelligence, Martinsried, Germany., D'Orazi FD; Department of Biological Structure, University of Washington, Seattle, WA, USA., Suzuki SC; Department of Biological Structure, University of Washington, Seattle, WA, USA., Godinho L; Institute of Neuronal Cell Biology, Technische Universität München, Munich, Germany., Lawrence O; Department of Biological Structure, University of Washington, Seattle, WA, USA., Rieke F; Department of Physiology and Biophysics, University of Washington, Seattle, WA, USA.; Vision Science Center, University of Washington, Seattle, WA, USA., Shekhar K; Department of Chemical and Biomolecular Engineering; Helen Wills Neuroscience Institute; Vision Sciences Graduate Program; California Institute of Quantitative Biosciences (QB3), University of California Berkley, Berkeley, CA, USA.; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA., Sanes JR; Department of Molecular and Cellular Biology, and Center for Brain Science, Harvard University, Cambridge, MA, USA., Baier H; Department Genes - Circuits - Behavior, Max Planck Institute for Biological Intelligence, Martinsried, Germany., Baden T; School of Life Sciences, University of Sussex, Brighton, UK.; Institute of Ophthalmic Research, University of Tübingen, Tübingen, Germany., Wong RO; Department of Biological Structure, University of Washington, Seattle, WA, USA., Yoshimatsu T; Department of Ophthalmology and Visual Sciences, Washington University in St Louis School of Medicine, St Louis, MO, USA. takeshi@wustl.edu.; BioRTC, Yobe State University, Damatsuru, Yobe, Nigeria. takeshi@wustl.edu.
Jazyk: angličtina
Zdroj: Nature ecology & evolution [Nat Ecol Evol] 2024 Jun; Vol. 8 (6), pp. 1165-1179. Date of Electronic Publication: 2024 Apr 16.
DOI: 10.1038/s41559-024-02404-w
Abstrakt: Vertebrates rely on rod photoreceptors for vision in low-light conditions. The specialized downstream circuit for rod signalling, called the primary rod pathway, is well characterized in mammals, but circuitry for rod signalling in non-mammals is largely unknown. Here we demonstrate that the mammalian primary rod pathway is conserved in zebrafish, which diverged from extant mammals ~400 million years ago. Using single-cell RNA sequencing, we identified two bipolar cell types in zebrafish that are related to mammalian rod bipolar cell (RBCs), the only bipolar type that directly carries rod signals from the outer to the inner retina in the primary rod pathway. By combining electrophysiology, histology and ultrastructural reconstruction of the zebrafish RBCs, we found that, similar to mammalian RBCs, both zebrafish RBC types connect with all rods in their dendritic territory and provide output largely onto amacrine cells. The wiring pattern of the amacrine cells postsynaptic to one RBC type is strikingly similar to that of mammalian RBCs and their amacrine partners, suggesting that the cell types and circuit design of the primary rod pathway emerged before the divergence of teleost fish and mammals. The second RBC type, which forms separate pathways, was either lost in mammals or emerged in fish.
(© 2024. The Author(s), under exclusive licence to Springer Nature Limited.)
Databáze: MEDLINE
Externí odkaz: