| Autor: |
Bhatt MR; Department of Chemistry and Biochemistry, University of Delaware Newark, Delaware 19716, United States., Ganguly HK; Department of Chemistry and Biochemistry, University of Delaware Newark, Delaware 19716, United States., Zondlo NJ; Department of Chemistry and Biochemistry, University of Delaware Newark, Delaware 19716, United States. |
| Jazyk: |
angličtina |
| Zdroj: |
Biochemistry [Biochemistry] 2024 May 07; Vol. 63 (9), pp. 1118-1130. Date of Electronic Publication: 2024 Apr 16. |
| DOI: |
10.1021/acs.biochem.3c00646 |
| Abstrakt: |
Acyl capping groups stabilize α-helices relative to free N-termini by providing one additional C═O i ···H i +4 -N hydrogen bond. The electronic properties of acyl capping groups might also directly modulate α-helix stability: electron-rich N-terminal acyl groups could stabilize the α-helix by strengthening both i / i + 4 hydrogen bonds and i / i + 1 n → π* interactions. This hypothesis was tested in peptides X-AKAAAAKAAAAKAAGY-NH 2 , where X = different acyl groups. Surprisingly, the most electron-rich acyl groups (pivaloyl and iso -butyryl) strongly destabilized the α-helix. Moreover, the formyl group induced nearly identical α-helicity to that of the acetyl group, despite being a weaker electron donor for hydrogen bonds and for n → π* interactions. Other acyl groups exhibited intermediate α-helicity. These results indicate that the electronic properties of the acyl carbonyl do not directly determine the α-helicity in peptides in water. In order to understand these effects, DFT calculations were conducted on α-helical peptides. Using implicit solvation, α-helix stability correlated with acyl group electronics, with the pivaloyl group exhibiting closer hydrogen bonds and n → π* interactions, in contrast to the experimental results. However, DFT and MD calculations with explicit water solvation revealed that hydrogen bonding to water was impacted by the sterics of the acyl capping group. Formyl capping groups exhibited the closest water-amide hydrogen bonds, while pivaloyl groups exhibited the longest. In α-helices in the PDB, the highest frequency of close amide-water hydrogen bonds is observed when the N-cap residue is Gly. The combination of experimental and computational results indicates that solvation (hydrogen bonding of water) to the N-terminal amide groups is a central determinant of α-helix stability. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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