An Undergraduate Course in CRISPR/Cas9-Mediated Gene Editing in Zebrafish.

Autor:	Srivastava R; Department of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa, USA., Davison CW; Department of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa, USA., Krull AG; Department of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa, USA., Entriken SM; Department of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa, USA., Zumbrock A; Department of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa, USA., Cortes Hidalgo MD; Department of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa, USA., Adair KJ; Department of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa, USA., Escherich AM; Department of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa, USA., Lara JN; Department of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa, USA., Neverman EC; Department of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa, USA., Hodnefield M; Department of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa, USA., McElligtot E; Department of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa, USA., Sandquist EJ; Department of Zoology, Weber State University, Weber, Utah, USA., Ogilvie C; The Graduate School, Montana State University, Bozeman, Montana, USA., Lafontant P; Biology Department, Grinnell College, Grinnell, Iowa, USA., Essner JJ; Department of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa, USA.
Jazyk:	angličtina
Zdroj:	Zebrafish [Zebrafish] 2024 Apr; Vol. 21 (2), pp. 162-170.
DOI:	10.1089/zeb.2023.0091
Abstrakt:	We have developed a one-credit semester-long research experience for undergraduate students that involves the use of CRISPR/Cas9 to edit genes in zebrafish. The course is available to students at all stages of their undergraduate training and can be taken up to four times. Students select a gene of interest to edit as the basis of their semester-long project. To select a gene, exploration of developmental processes and human disease is encouraged. As part of the course, students use basic bioinformatic tools, design guide RNAs, inject zebrafish embryos, and analyze both the molecular consequences of gene editing and phenotypic outcomes. Over the 10 years we have offered the course, enrollment has grown from less than 10 students to more than 60 students per semester. Each year, we choose a different gene editing strategy to explore based on recent publications of gene editing methodologies. These have included making CRISPants, targeted integrations, and large gene deletions. In this study, we present how we structure the course and our assessment of the course over the past 3 years.
Databáze:	MEDLINE
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